Remodeling And Spacing Factor (RSF)‐1, Transforming Growth Factor β Receptor (TGFBR)‐2, ACVR‐1, Tumor Necrosis Factor Superfamily Member (TNFSF)‐9, and Von Willebrand Factor (VWF) proteins were selected for validation, and their expression in EVs and MSCs (n = 5 each) measured by Western blot (Abcam, ab83322, 1:1,000; Abcam, ab61213, 1:1,000; lsbio, LS‐B3020, 1:1,000; Abcam, ab64912, 1:1,000; and Abcam, ab6994, 1:500, respectively).
Ab64912
Manufactured by Abcam
Sourced in United States
Ab64912 is a lab equipment product. It serves a core function, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
2 protocols using ab64912
1
Validation of EV and MSC Protein Expression
2
Immunohistochemical Analysis of CD137 and CD137L
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The 4 µm thick paraffin-embedded formalin-fixed tissue sections were de-paraffinized, and heat-based antigen retrieval was performed in 0.1 M citrate buffer (pH 6.0). Endogeneous peroxidase activity was inhibited using hydrogen peroxide. The primary antibodies for CD137 (ab3169) and CD137L (ab64912) were purchased from Abcam (Cambridge, MA, USA). The detection system was the streptavidin-biotin-peroxidase complex technique (ABC kit; Vector Laboratories, Burlingame, CA, USA) with diaminobenzidine (DAB; Nichirei Bioscience, Tokyo, Japan) as the chromogen. In situ hybridization (ISH) of Epstein–Barr virus-encoded mRNA (EBER) was performed for detection of EBV in tissue sections by Epstein-Barr Virus (EBER) PNA Probe/Fluorescein (DAKO, Carpinteria, CA, USA) and second antibody for Fluorescein (Dako, Glostrup, Denmark).
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