HSA (≥96%, free fatty acid), β-CyD, β-D(+) glucose, sodium Alg and sodium bicinchoninate (BCA) were purchased from Sigma (USA). Membrane filters (0.2 μm pore size, 25 mm in diameter) and dialysis tubings (cut off 10,000 MW) were from Whatman (UK). Tre, DTT, Tris buffer and sodium azide were from Merck (Germany). All other materials were of analytical grade. All solutions were prepared with deionized water.
Sodium alg
Manufactured by Merck Group
Sourced in United States
Sodium Alg is a water-soluble, gelling agent derived from brown seaweed. It is commonly used as a thickening, stabilizing, and gelling agent in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Membrane filter, by Cytiva (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Tris buffer, by Merck Group (1 mentions)
β d glucose, by Merck Group (1 mentions)
β cyd, by Merck Group (1 mentions)
Whatman, by Cytiva (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Poloxamer 407, by Merck Group (1 mentions)
Acetonitrile, by RCI Labscan (1 mentions)
Cacl2, by Merck Group (1 mentions)
Bile salt, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
Pancreatin, by Merck Group (1 mentions)
2 2 bipyridine, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
6 protocols using sodium alg
1
Synthesis and Characterization of Protein Conjugates
2
Turmerone-Chitosan Nanoparticles for Breast Cancer
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Chemicals. TO was purchased from Thai–China Flavours and Fragrances Industry (Nonthaburi, Thailand). The amount of ar-turmerone in TO was found to be about 12%. Ar-turmerone was provided by the Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University (Bangkok, Thailand). CS (MW = 63 kDa, 91.74% DD) was supplied by Marine Bio-Resources (Samut Sakorn, Thailand). Sodium Alg (medium viscosity) and poloxamer 407 were purchased from Sigma-Chemicals (St. Louis, MO, USA). Acetonitrile was purchased from RCI Labscan (Bangkok, Thailand). Absolute ethanol, glacial acetic acid, calcium chloride, and other chemicals were purchased from Carlo Erba reagents (Val de Reuil, France).
Cell Culture. Human breast cancer cells (MCF-7 and MDA-MB-231) and HEK293 were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum and 100 units/mL penicillin/streptomycin (Gibco™ Thermo Fisher Scientific Inc., Waltham, MA, USA) in humidified atmosphere of 5% CO2 at 37 °C.
Cell Culture. Human breast cancer cells (MCF-7 and MDA-MB-231) and HEK293 were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum and 100 units/mL penicillin/streptomycin (Gibco™ Thermo Fisher Scientific Inc., Waltham, MA, USA) in humidified atmosphere of 5% CO2 at 37 °C.
3
Alginate Microgel Crosslinking and Preparation
4
Wheat Germ Flour Bioactive Compounds
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All chemical reagents and microbial media used in this study were purchased with analytical grade. The studied foodborne bacteria and fungi were also supplied from American type culture collection (ATCC). Wheat germ flour (containing 26.39, 2.82, 11.17, 8.90 and 50.72% protein, ash, moisture, fat and total carbohydrates, respectively) as a by-product of wheat milling was purchased from a local milling industry. Sodium Alg (with molecular weight of 100 KDa), Ch (with medium molecular weight), DPPH, OTA, acetonitrile, isopropanol, pure phytate, ammonium iron III, 2, 2′-bipyridine, bile salt, pepsin and pancreatin were also supplied from Sigma-Aldrich (Burlington, VT, USA).
5
Probiotic Strain Characterization
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University Putra Malaysia (UPM) provided the S. agalactiae (ATCC 13813). Fk and PG were purchased locally (Iran). Sodium hydrogen phosphate, hydrochloric acid, calcium chloride, sodium hydroxide, de Man Rogosa Sharpe (MRS) broth, MRS agar, and Acrylamide (AAm) (Merck, Germany) and N, N-methylene bis-acrylamide (NN-MBAAm), ammonium persulphate (APS), fructooligosaccharides (FOS), oxgall, and sodium ALG were purchased (Sigma-Aldrich, Germany).
6
Biopolymer-based Biomaterial Synthesis
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CH (230 KDa average molecular weight and 80.6% of N-deacetylation) was supplied by Aldrich Chemical Co. (Milwaukee, WI, USA), GA (Mw = 250KDa), GEL (type A from pork skin, 125 Bloom value) and MN pharmaceutical grade were purchased from Parafarm, (Buenos Aires, Argentina). Sodium ALG (Sigma-Aldrich Co. Buenos Aires, Argentina), CB (Carbopol ® 971NF) by Lubrizol Advanced Materials, Inc. (Cleveland, OH, USA). All other chemicals were of analytical grade.
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