A431 cells (CRL-1555) and HeLa cells (CCL-2) were purchased from the American Type Culture Collection. Dulbecco’s modified Eagle’s medium (DMEM; 10-014-CV), Eagle’s minimum essential medium (EMEM; 10-010-CV), phosphate-buffered saline (PBS; 21-014-CV), Dulbecco’s PBS (DPBS; 21-030 and 21-031), 0.25% trypsin/0.53 mM EDTA, and 0.25% trypsin/2.21 mM EDTA were purchased from Corning CellGro. MEM nonessential amino acids (11140), DMEM (no phenol red, 21063), and Hepes (15630-080) were purchased from Gibco. Micropipette preparation glass-bottom culture dishes (P50G-0-14-F) were purchased from MatTek Corporation. XenoWorks Microinjection Systems, P-1000 pipette puller, and borosilicate glass tubes (BF100-78-10) were used for microinjections (Sutter Instrument). Cetuximab and matuzumab antibodies were from Merck. VHH nanobodies binding to EGFR (EgA1 and EgB4) were produced as described elsewhere (57 (link)). Peptides: CPP, R9GGLAAibSGWKH6; Tb-pep, TbL4-GSGAAAGLS-H6; AF-peptide, AF-CSTRIDEANQAATSLP7SH6). QDs emitting at 625 nm were the same as described previously (36 (link)–38 (link), 45 (link)). These were made biocompatible by cap exchange with dihydrolipoic acid–modified compact ligand 4 (DHLA-CL4) or DHLA-PEG-methoxy (36 (link), 44 ). See fig. S7 for ligand structures. Lumi4-Tb reagents were provided by Lumiphore Inc.
Cetuximab and matuzumab antibodies
Manufactured by Merck Group
Cetuximab and matuzumab are monoclonal antibodies produced by Merck Group for use in research and laboratory settings. These antibodies are designed to target specific proteins or receptors on cells, allowing researchers to study their function and interactions. The core function of these antibodies is to serve as tools for scientific investigation, without any interpretation or extrapolation regarding their intended use.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cetuximab and matuzumab antibodies
1
Biocompatible Quantum Dots and Peptides for Cell Signaling Studies
2
Yeast Surface Display of Antibodies
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The Saccharomyces cerevisiae strain EBY100 was utilized for yeast surface display33 (link). YPD medium was composed of 20 g/L tryptone, 20 g/L dextrose and 10 g/L yeast extract. SD-CAA medium was prepared using 6.8 g/L yeast nitrogen base without amino acids but supplemented with ammonium sulphate, 5 g/L Bacto Casamino Acids, 20 g/L dextrose, 8.6 g/L NaH2PO4 × H2O, and 5.4 g/L Na2HPO4. SG-CAA medium was prepared similarly except for the substitution of dextrose sugar with galactose. Phosphate-buffered saline (PBS) contained 8.1 g/L NaCl, 0.75 g/L KCl, 1.13 g/L Na2HPO4 and 0.27 g/L KH2PO4. Cetuximab and matuzumab antibodies were kindly provided by Merck.
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