NTCU and LPS were purchased from Toronto Research Chemicals (Toronto, Canada) and
Sigma (St. Louis, MO), respectively. BioResponse diindolylmethane (DIM) was kindly
provided by Dr. Michael Zeligs (BioResponse, LLC). Anti-phospho-STAT3, anti-total STAT3,
anti-phospho-Akt, anti-total Akt, anti-phospho-extracellular signal-regulated kinase
(ERK), anti-total ERK, anti-phospho-p38, total p-38, anti-Mcl-1, anti-p53, anti-COX2,
anti-phospho IκBα, anti-total IκBα, anti-Bax, anti-p-21,
anti-PARP, anti-β-actin and goat anti-rabbit IgG secondary antibody were from Cell
Signaling Technology (Beverly, MA). Mouse diets (AIN-93G and AIN-93M) were purchased from
Harlan Teklad (Madison, WI). These diets are standard diets for lung tumorigenesis studies
in A/J mice. The AIN-93G diet, high in protein and fat, was used to support rapid growth
of the mice until eight weeks of age. AIN-93G diet was then replaced by AIN-93M diet, a
low-protein and low-fat diet, which is recommended for adult maintenance.
Sigma (St. Louis, MO), respectively. BioResponse diindolylmethane (DIM) was kindly
provided by Dr. Michael Zeligs (BioResponse, LLC). Anti-phospho-STAT3, anti-total STAT3,
anti-phospho-Akt, anti-total Akt, anti-phospho-extracellular signal-regulated kinase
(ERK), anti-total ERK, anti-phospho-p38, total p-38, anti-Mcl-1, anti-p53, anti-COX2,
anti-phospho IκBα, anti-total IκBα, anti-Bax, anti-p-21,
anti-PARP, anti-β-actin and goat anti-rabbit IgG secondary antibody were from Cell
Signaling Technology (Beverly, MA). Mouse diets (AIN-93G and AIN-93M) were purchased from
Harlan Teklad (Madison, WI). These diets are standard diets for lung tumorigenesis studies
in A/J mice. The AIN-93G diet, high in protein and fat, was used to support rapid growth
of the mice until eight weeks of age. AIN-93G diet was then replaced by AIN-93M diet, a
low-protein and low-fat diet, which is recommended for adult maintenance.