Anti igd pe

Manufactured by Southern Biotech

Anti-IgD-PE is a fluorescently-labeled antibody that binds to the IgD protein. It is used in flow cytometry applications to identify and quantify cells expressing IgD on their surface.

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IgD PE (9 citations) Anti-IgD (3 citations)

2 protocols using anti igd pe

Multi-color Flow Cytometry Protocol

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For cell surface staining the following mAbs were used (from BD Biosciences unless otherwise stated): Anti-CD3-FITC (145-2C11), anti-CD4-APC (RM4-5), anti-CD19-APC (1D3), anti-CD19-Pacific Blue (1D3, eBioscience), anti-CD43-Biotin (S7) detected with SA-Alexa-647 (Invitrogen), anti-CD43-FITC (S7), anti-CD44-FITC (IM7), anti-CD45-PercP (30F11), anti-CD69-PE (H1.2F3), anti-CD79 (polyclonal rabbit) detected with goat-anti-rabbit IgG-Alexa-649 (Molecular Probes), anti-CD79b-DyLight-488 (HM79), anti-CD79b-Dylight-649 (HM79), anti-CD80-PE (1610A1), anti-CD86-PE (GL-1), anti-B220-PerCP (RA3-6B2), anti-FcγRIIB-Dylight-488 (2.4G2), anti-Hamster IgG FITC (G70-204/G94.56), anti-IA/IE FITC (2G9), anti-IgD-PE (11-26, Southern Biotech), anti-IgM-Dylight-649 (b-7-6), anti-MHC-II-Dylight-488 (D3.137), and anti-PNA FITC (Sigma). Cells were incubated with Fc block (BD Biosciences) and stained in FACS buffer (PBS+1% BSA + 0.02% NaN3) to reduce non-specific staining. Flow cytometry was performed on cells using a CYAN flow cytometer (Beckman Coulter) or FACS Calibur flow cytometer (BD Biosciences) and analyzed using FlowJo (Treestar), CellQuestPro software (BD Biosciences) and PRISM (Graphpad Software).

Hardy I.R., Anceriz N., Rousseau F., Seefeldt M.B., Hatterer E., Irla M., Buatois V., Chatel L.E., Getahun A., Fletcher A., Cons L., Pontini G., Hertzberg N.A., Magistrelli G., Malinge P., Smith M.J., Reith W., Kosco-Vilbois M.H., Ferlin W.G, & Cambier J.C. (2014). Anti-CD79 Antibody Induces B cell Anergy That Protects Against Autoimmunity. Journal of immunology (Baltimore, Md. : 1950), 192(4), 1641-1650.

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Comprehensive Immunophenotyping of Immune Cells

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Anti-CD19 APC-Cy7, anti-CD38 PerCp-Cy5.5, anti-CD4 PE-Cy7, anti-CD25 PerCP-Cy5.5, anti-CD86 APC, anti-CD80 BV421, anti-CD45RA BV605, anti-ICOS PE, anti-CD21 PE-Cy7, anti-CD80 BV421, anti-CD27 PerCp-Cy5.5, anti-PD-1 APC, anti-CD45RA APC-Cy7, anti-HLA-DR BV605, anti-CD11c AF700, anti-CD86 BV711, anti-CD95 BV650, anti-IgD BV786, anti-FCRL5 APC, anti-CXCR3 BV421, anti-CCR6 BV605, anti-CD19 BV650, anti-CD19 BV605, anti-PD-L1 BV421, anti-PD-L2 PE, anti-ICOS-L PE, anti-OX40L PE, anti-IL-10 PE, anti-IL-10 APC, anti-TNF-α APC-Cy7 (all obtained from BioLegend); anti-IgD FITC and anti-IgD PE (both from Southern Biotech); anti-CD27 PE (Dako); and anti-CD21 PE-Cy7, anti-CD69 FITC, anti-HLA-DR FITC, anti-CD27 BV605, anti-CD40 FITC, anti-CD40L PE, anti-ICAM-1 PE, anti-CXCR5 BUV395 and anti-CD27 BUV395 (all from BD Biosciences).

Reincke M.E., Payne K.J., Harder I., Strohmeier V., Voll R.E., Warnatz K, & Keller B. (2020). The Antigen Presenting Potential of CD21low B Cells. Frontiers in Immunology, 11, 535784.

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