The complementary DNA fragment of the 3′ untranslated region (UTR) of the wild-type or mutant LINC01134 fragment was subcloned downstream of luciferase in the pGL3-Basic luciferase reporter gene (Promega, Beijing, China). The firefly luciferase gene containing wild-type or mutant LINC01134 fragment was cotransfected with an empty vector or the miR-140-3p mimic. After 48 h post-transfection, luciferase activity was measured using the Dual-Luciferase Kit (Promega, Beijing, China). The binding between WNT5A and miR-140-3p was validated using the same method.
Pgl3 basic luciferase reporter gene
Manufactured by Promega
Sourced in China
The PGL3-Basic luciferase reporter gene is a plasmid vector that contains the firefly luciferase gene as a reporter. It is designed for use in gene expression studies and allows for the measurement of promoter activity in cell-based assays.
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Lab products found in correlation
2 protocols using pgl3 basic luciferase reporter gene
1
Investigating miR-140-3p Regulation of LINC01134
2
Luciferase Assay for DUXAP8 and MAPK1 Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The complementary DNA fragment containing wild-type or mutant DUXAP8 fragment/MAPK1 3′ untranslated region (UTR) was subcloned to the downstream of luciferase in the pGL3-basic luciferase reporter gene (Promega, Beijing, China). The firefly luciferase gene containing wild-type or mutant DUXAP8 fragment/MAPK1 was cotransfected with an empty vector or miR-584-5p mimics, and luciferase activity was measured using the Dual-Luciferase Kit (Promega, Beijing, China) 48 h after transfection.
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