The largest database of trusted experimental protocols

Milli q reverse osmosis

Manufactured by Merck Group
Sourced in United States

Milli-Q reverse osmosis is a lab equipment product that purifies water by using a semi-permeable membrane to remove unwanted contaminants and minerals. The core function of this product is to provide high-quality, ultrapure water for use in various laboratory applications.

Automatically generated - may contain errors

3 protocols using milli q reverse osmosis

1

UPLC-MS Analysis of Chemical Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Waters Acquity TQD UPLC/ Mass spectrometer (Waters Co., Milford, MA, USA) was used in the study. Other equipment used included vortex mixer Taboys® model AP 56 (TROEMNER. Hingham, MA, USA), analytical balance Mettler Toledo® model XS 205 (Greifensee, Switzerland), and a sample concentrator Thermo® Savant SC210A speed Vac (Waltham, MA, USA). Deionized water was prepared by Milli-Q reverse osmosis (Millipore®, Bedford, MA, USA).
+ Open protocol
+ Expand
2

Synthesis and Characterization of 3,4,3-LI(1,2-HOPO) Ligand

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ligand 3,4,3-LI(1,2-HOPO) was synthesized and characterized by Ash Stevens, Inc. (Detroit, MI) as previously described.4 (link) Purity was determined to be 97.3% by high performance liquid chromatography (HPLC) analysis (data not shown). All other chemicals were obtained from commercial suppliers and used as received. Purified deionized water, using Millipore Milli-Q reverse osmosis, was used to prepare aqueous solutions. Pooled plasma samples and male and female liver microsomes from Sprague Dawley rat, beagle dog, and human were purchased from Bioreclamation, Inc. (Hicksville, NY). All samples were stored at −80°C until use. Either [13C4]-3,4,3-LI(1,2-HOPO), with a chemical purity of 99.3% (Moravek Biochemicals, Inc., Brea, CA), or ethyl nicotinate (Sigma Chemicals, St. Louis, MO) was used as the internal standard during the liquid chromatography coupled with mass spectrometric (LC-MS or LC-MS/MS) analyses.
+ Open protocol
+ Expand
3

Chromatographic Analysis of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
HPLC-grade acetonitrile and formic acid were acquired from J. T. Baker (Brazil) and LC-MS grade methanol (from Chromasolv) was used as mobile-phase components in the chromatographic analysis. Deuterated solvents were acquired from Euriso-Top (Saint Aubin, France). Folin-Ciocalteu reagent, Triton X-100, Yeast Nitrogen Base medium (YNB), D-glucose monohydrated, XTT/menadione, and fluconazole were purchased from Sigma-Aldrich (St. Louis, Missouri, United States of America). Mueller-Hinton medium was acquired from HiMedia (Mumbai, Maharashtra, India). Sabouraud Dextrose Agar (SDA) was acquired from Basingstoke, Hampshire, United Kingdom. RPMI 1640 culture medium (Roswell Park Memorial Institute) was acquired from Angus buffers and Biochemical, Niagara Falls, New York, United States of America. All other reagents and solvents used in the experiments were of analytical grade. The water used was purified by Milli-Q reverse osmosis (Millipore).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!