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Lb broth

Manufactured by Scharlab
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Sourced in Spain

LB Broth is a commonly used culture medium for the growth of various bacteria, including Escherichia coli. It provides the necessary nutrients and growth factors to support the cultivation of these microorganisms.

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Lab products found in correlation

Glycerol, by Merck Group (1 mentions) Tryptic soy broth (tsb), by Scharlab (1 mentions) Tryptic soy agar tsa, by Scharlab (1 mentions)

2 protocols using lb broth

1

Isolation and Storage of Bacterial Symbionts from G. mellonella

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Bacterial symbiont was isolated from hemolymph of G. mellonella larvae infected with the EPNs recovered, following the procedure described by Kazimierczak et al. (2017) (link). After isolation, bacterial symbionts were stored at −80°C in LB Broth (Scharlab, Spain) with 20% (v/v) glycerol (Merck, United States) and deposited in the Microorganisms Collection of Pontificia Universidad Javeriana – CMPUJ.
Neira-Monsalve E., Wilches-Ramírez N.C., Terán W., del Pilar Márquez M., Mosquera-Espinosa A.T, & Sáenz-Aponte A. (2020). Isolation, identification, and pathogenicity of Steinernema carpocapsae and its bacterial symbiont in Cauca-Colombia. Journal of Nematology, 52, e2020-89.
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2

Maintenance and Cultivation of Bacterial Strains

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Pseudomonas aeruginosa PAO1 (ATCC 15692) and Staphylococcus aureus SA31 (ATCC 29213) were maintained in initial cryo-stock cultures at −80°C, and they resuscitated in Luria-Bertani (LB) agar (Scharlab, S.L., Spain) and Tryptic Soy Agar (TSA) (Scharlab, S.L., Spain) plates, respectively. Isolated colonies were grown overnight in LB broth and Tryptic Soy Broth (TSB) (Scharlab, S.L., Spain), respectively, at 37°C with shaking at 200 rpm. Unless otherwise specified, all reagents were purchased from Sigma, Spain.
Rubio-Canalejas A., Baelo A., Herbera S., Blanco-Cabra N., Vukomanovic M, & Torrents E. (2022). 3D spatial organization and improved antibiotic treatment of a Pseudomonas aeruginosa–Staphylococcus aureus wound biofilm by nanoparticle enzyme delivery. Frontiers in Microbiology, 13, 959156.
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