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Tigit pe

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TIGIT PE is a fluorescent-labeled antibody that binds to the TIGIT (T-cell immunoreceptor with Ig and ITIM domains) protein. TIGIT is an inhibitory receptor expressed on T cells and NK cells that negatively regulates their activation and function.

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Lab products found in correlation

Cd45ra percp cy5, by BioLegend (1 mentions) Pd1 apc, by BioLegend (1 mentions) Ficoll paque plus density gradient media, by GE Healthcare (1 mentions) Facsaria 3, by BD (1 mentions) Lsrfortessa x 20, by BD (1 mentions) Fixable viability dye efluor 506, by Thermo Fisher Scientific (1 mentions) Cd8a fitc, by BioLegend (1 mentions) Pe cy7 anti human cd4, by BioLegend (1 mentions) Tim 3 pe, by BD (1 mentions) Ctla 4 pe, by BD (1 mentions) Cd4 buv737, by BD (1 mentions) Pd1 fitc, by BD (1 mentions) Cd3 percp, by BD (1 mentions) Cytofix, by BD (1 mentions) Cd8 v500, by BD (1 mentions)

Spelling variants of this product

TIGIT-PE/Cy7 (10 citations) Anti-TIGIT-PE-Cy7 (2 citations) Anti-human TIGIT-PE (2 citations) TIGIT-PE-Cy5 (2 citations)

2 protocols using tigit pe

1

Multiparametric Flow Cytometry of PBMCs

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Peripheral blood mononuclear cells (PBMCs) were separated by density-gradient centrifugation using Ficoll-Paque PLUS density-gradient media (GE Healthcare, Uppsala, Sweden). PBMCs or CSF specimens were stained with anti-human CD4 PE/Cy7, CD8a-FITC, CD45RA-PerCP/Cy5.5, CD27-Brilliant Violet, TIGIT PE, PD-1 APC antibodies (BioLegend, San Diego, CA), and CD3− PerCP/Cy5.5 antibodies (BD Biosciences, Franklin Lakes, NJ) for flow cytometry. Data were obtained using fluorescence-activated cell sorting (FACS) Verse LSRFortessa X-20 or FACS Aria III (BD Biosciences). Dead cells were excluded using Fixable Viability Dye eFluor 506 (Thermo Fisher Scientific, Waltham, MA). Negative expression was defined as fluorescence minus one control (eFigure 1, links.lww.com/NXI/A710).
Koto S., Chihara N., Akatani R., Nakano H., Hara A., Sekiguchi K., Matsumoto R, & Toda T. (2022). Transcription Factor c-Maf Promotes Immunoregulation of Programmed Cell Death 1–Expressed CD8+ T Cells in Multiple Sclerosis. Neurology® Neuroimmunology & Neuroinflammation, 9(4), e1166.
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2

T-Cell Checkpoint Marker Analysis

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Cells were collected at the end of the study and assessed for T-Cell checkpoint markers (BD, LSRII). The following antibodies and fluorophores were used: CD3 (PerCP, BD), CD4 (BUV737, BD), CD8 (V500, BD), CTLA4 (PE, BD), Lag3 (APC-R700, BD), PD1 (FITC, BD), TIGIT (PE, BioLegend), and TIM3 (PE, BD). Cells were fixed post staining (Cytofix, BD).
Li M., Chin L.Y., Shukor S., Tamayo A.G., Maus M.V, & Parekkadan B. (2019). Effects of Intermittent T-Cell Cluster Disaggregation on Proliferative Capacity and Checkpoint Marker Expression. Autoimmunity, 52(3), 102-107.
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