Ham s f12 nutrient medium

The ccRCC (786-0) cell line with CAIX antigen overexpressed was obtained from the Key Laboratory for Cancer Biology Treatment of Xuzhou Medical College as a gift. EA.hy926 normal human umbilical vein endothelial cell line (HUVEC) that is absent of CAIX antigen expression and human lung fibroblast HLF-1 cells were kindly provided by Teng Fei (Laboratory of Medical Imaging of Xuzhou Medical College). The original source of these three kinds of cell lines was from the same commercial source, the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). NIH-3T3 mouse fibroblast cells were also purchased from it. The 786-0 renal carcinoma cells were cultured in a 10% FBS-containing RPMI 1640 medium (Gibco, Grand Island, NY) supplemented with penicillin (100 µg/mL), and streptomycin (100 µg/mL). NIH-3T3 and HUVEC cells were propagated in 10% FBS-containing DMEM medium (Gibco, Grand Island, NY) supplemented with penicillin (100 µg/mL), and streptomycin (100 µg/mL). HLF-1 cells were cultured in Ham’s F12 nutrient medium (Gibco, Grand Island, NY) supplemented with 10% FBS, penicillin (100 µg/mL), and streptomycin (100 µg/mL). All cells were grown in a humidified incubator (Thermo, USA) at 37°C under 5% CO₂ atmosphere.

As described [8 (link),9 (link)], colorectal cancer cell lines, including HT-29, DLD-1 and Caco-2, were purchased from the cell bank of Chinese Academy of Science (CAS) Shanghai Biological Institute (Shanghai, China). Cells were maintained in FBS-containing RPMI/DMEM medium. Human NCM460 colon epithelial cell line was provided by Fudan IBS Cell center (Shanghai, China). Cells were cultured in Ham's F12 nutrient medium (Gibco) [19 (link)].