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Ab19569

Manufactured by Abcam
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Ab19569 is an antibody that recognizes the protein target. It is suitable for use in various laboratory applications. The product details and intended use are available on the Abcam website.

Automatically generated - may contain errors

Lab products found in correlation

Ab6326, by Abcam (2 mentions) Ve cadherin, by Santa Cruz Biotechnology (2 mentions) Mca1957, by Bio-Rad (2 mentions) Sc 17320, by Santa Cruz Biotechnology (2 mentions) Be0027, by BioXCell (2 mentions) Be0088, by BioXCell (2 mentions) Mab360mi, by Vector Laboratories (2 mentions) Ab2218, by Bio-Rad (2 mentions) Mab002 clone 11711, by BioXCell (2 mentions) Sc 8066, by Santa Cruz Biotechnology (2 mentions) Biotinylated anti rabbit secondary antibody, by Vector Laboratories (2 mentions) Vectastain dab 3 3 diamino benzidine kit, by Vector Laboratories (2 mentions) Qimaging digital camera, by Nikon (2 mentions) A568 conjugated anti rabbit antibody, by Thermo Fisher Scientific (2 mentions) A488 conjugated anti rat antibody, by Thermo Fisher Scientific (2 mentions) Tcs sp8x white light laser confocal system, by Leica camera (2 mentions) Tween 20, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Vectastain abc kit, by Vector Laboratories (2 mentions) Eclipse e800 microscope, by Nikon (2 mentions)

5 protocols using ab19569

1

Multimodal Immunofluorescence Staining Protocol

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Rat monoclonal anti-BrdU (1:500, Abcam, ab6326, clone BU1/75[ICR1])
Rat monoclonal anti-VCAM1 (1:125, Abcam, ab19569, clone M/K-2)
Goat monoclonal anti-Sox2 (1:100, Santa Cruz, sc-17320, clone Y-17)
Goat polyclonal anti-doublecortin (DCX) (1:100, Santa Cruz, sc-8066, clone C-18)
Goat polyclonal VE-Cadherin (Santa Cruz Biotechnology, sc-6458, clone C-19)
Click-iT® Plus EdU Alexa Fluor® 488 Imaging Kit (Thermo/Life Technologies, C-10637)
Mouse monoclonal anti-GFAP (1:1000, Chemicon/Fisher, MAB360MI, clone GA5)
DyLight 488 Lectin (1:200, Vector, DL-1174)
Rabbit monoclonal anti-Aquaporin 4 (1:500, Millipore, AB2218, clone)
Rat monoclonal anti-CD68 (1:600, Serotec, MCA1957, clone FA-11)
Rabbit polyclonal anti-Iba1 (1:250, ProteinTech, 10904–1-AP)
Mouse anti-human-VCAM1 antibody (Novus Biologicals, BBA5, clone BBIG-V1)
Mouse monoclonal anti-human IgG antibody (R&D Systems, MAB002 clone 11711)
Rat monoclonal anti-VCAM-1 (BioxCell, BE0027, clone M/K-2.7)
Rat IgG1 Isotype antibody (BioxCell, BE0088, clone HRPN)
Yousef H., Czupalla C.J., Lee D., Chen M.B., Burke A.N., Zera K.A., Zandstra J., Berber E., Lehallier B., Mathur V., Nair R.V., Bonanno L.N., Yang A.C., Peterson T., Hadeiba H., Merkel T., Körbelin J., Schwaninger M., Buckwalter M.S., Quake S.R., Butcher E.C, & Wyss-Coray T. (2019). Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nature medicine, 25(6), 988-1000.
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2

Tumor Immunohistochemistry and Immunofluorescence

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Tumor samples were fixed overnight in 4% PFA/PBS, washed with PBS and a small part was immersed in 30% sucrose/PBS, embedded in OCT (VWR Chemicals) and frozen, while the rest was embedded in paraffin. 4-μm-thick sections of FFPE (Formalin-Fixed, Paraffin-Embedded) tumors were blocked using 1% BSA in TBS containing 0.05% Tween 20 (Sigma) (TTBS) and incubated with anti-Ki67 (Thermofisher Scientific, MA5-14520) or anti-CD31 (Abcam, ab28364) primary antibodies. A biotinylated secondary anti-rabbit antibody (Vector Laboratories) and the Vectastain ABC kit (Vector Laboratories) were used for signal detection and amplification, respectively. Signal development was performed with the Vectastain DAB (3,3-diamino-benzidine) kit (Vector Laboratories) and hematoxylin was used as a counterstain. Images were acquired with an Eclipse E800 microscope (Nikon) equipped with a QImaging Digital Camera. Cryosections were incubated with the anti-CD31 and anti-VCAM1 (Abcam, ab19569) antibodies, followed by the secondary A568-conjugated anti–rabbit antibody (Invitrogen, A110011) and the A488-conjugated anti-rat antibody (Invitrogen, A11006), respectively. Mounting medium containing DAPI (Sigma-Aldrich) was used to stain the nuclei and images were acquired using a Leica TCS SP8X White Light Laser confocal system. Quantifications were performed using ImageJ software analysis
Hatzioannou A., Banos A., Sakelaropoulos T., Fedonidis C., Vidali M.S., Köhne M., Händler K., Boon L., Henriques A., Koliaraki V., Georgiadis P., Zoidakis J., Termentzi A., Beyer M., Chavakis T., Boumpas D., Tsirigos A, & Verginis P. (2019). An intrinsic role of IL-33 in Treg cell-mediated tumor immunoevasion. Nature immunology, 21(1), 75-85.
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3

Multimodal Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rat monoclonal anti-BrdU (1:500, Abcam, ab6326, clone BU1/75[ICR1])
Rat monoclonal anti-VCAM1 (1:125, Abcam, ab19569, clone M/K-2)
Goat monoclonal anti-Sox2 (1:100, Santa Cruz, sc-17320, clone Y-17)
Goat polyclonal anti-doublecortin (DCX) (1:100, Santa Cruz, sc-8066, clone C-18)
Goat polyclonal VE-Cadherin (Santa Cruz Biotechnology, sc-6458, clone C-19)
Click-iT® Plus EdU Alexa Fluor® 488 Imaging Kit (Thermo/Life Technologies, C-10637)
Mouse monoclonal anti-GFAP (1:1000, Chemicon/Fisher, MAB360MI, clone GA5)
DyLight 488 Lectin (1:200, Vector, DL-1174)
Rabbit monoclonal anti-Aquaporin 4 (1:500, Millipore, AB2218, clone)
Rat monoclonal anti-CD68 (1:600, Serotec, MCA1957, clone FA-11)
Rabbit polyclonal anti-Iba1 (1:250, ProteinTech, 10904–1-AP)
Mouse anti-human-VCAM1 antibody (Novus Biologicals, BBA5, clone BBIG-V1)
Mouse monoclonal anti-human IgG antibody (R&D Systems, MAB002 clone 11711)
Rat monoclonal anti-VCAM-1 (BioxCell, BE0027, clone M/K-2.7)
Rat IgG1 Isotype antibody (BioxCell, BE0088, clone HRPN)
Yousef H., Czupalla C.J., Lee D., Chen M.B., Burke A.N., Zera K.A., Zandstra J., Berber E., Lehallier B., Mathur V., Nair R.V., Bonanno L.N., Yang A.C., Peterson T., Hadeiba H., Merkel T., Körbelin J., Schwaninger M., Buckwalter M.S., Quake S.R., Butcher E.C, & Wyss-Coray T. (2019). Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nature medicine, 25(6), 988-1000.
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4

Blocking Endothelial Molecules in Malaria

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To investigate the effects of blocking various molecules present in the vascular endothelium, antibodies used included the following: RB40.34 (rat IgG1, 30 μg per mouse; BD Biosciences, catalog no. 553742), a monoclonal antibody (mAb) against murine P-selectin and 9A9 (rat IgG1, 30 μg per mouse), a mAb against murine E-selectin (provided by K. Ley, La Jolla Institute for Allergy and Immunology) or alternatively a mAb against murine E-selectin (rat IgG1, clone 96419, 30 μg per mouse; R&D Systems no. MAB5751-100). A 1:1 combination of P- and E-selectin antibodies was also used. Likewise, rat mAbs YN1/1.7.4 directed against ICAM-1 (50 μg per mouse, Abcam no. ab25375), rat mAb MK2 directed against VCAM-1 (50 μg per mouse, Abcam no. ab19569), or a 1:1 combination of YN1 and MK2 were used. Antibodies were injected intravenously (E-selectin and P-selectin) or intraperitoneally (all other antibodies) for two consecutive days before injection of purified schizonts/merozoites.
De Niz M., Meibalan E., Mejia P., Ma S., Brancucci N.M., Agop-Nersesian C., Mandt R., Ngotho P., Hughes K.R., Waters A.P., Huttenhower C., Mitchell J.R., Martinelli R., Frischknecht F., Seydel K.B., Taylor T., Milner D., Heussler V.T, & Marti M. (2018). Plasmodium gametocytes display homing and vascular transmigration in the host bone marrow. Science Advances, 4(5), eaat3775.
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5

Tumor Immunohistochemistry and Immunofluorescence

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumor samples were fixed overnight in 4% PFA/PBS, washed with PBS and a small part was immersed in 30% sucrose/PBS, embedded in OCT (VWR Chemicals) and frozen, while the rest was embedded in paraffin. 4-μm-thick sections of FFPE (Formalin-Fixed, Paraffin-Embedded) tumors were blocked using 1% BSA in TBS containing 0.05% Tween 20 (Sigma) (TTBS) and incubated with anti-Ki67 (Thermofisher Scientific, MA5-14520) or anti-CD31 (Abcam, ab28364) primary antibodies. A biotinylated secondary anti-rabbit antibody (Vector Laboratories) and the Vectastain ABC kit (Vector Laboratories) were used for signal detection and amplification, respectively. Signal development was performed with the Vectastain DAB (3,3-diamino-benzidine) kit (Vector Laboratories) and hematoxylin was used as a counterstain. Images were acquired with an Eclipse E800 microscope (Nikon) equipped with a QImaging Digital Camera. Cryosections were incubated with the anti-CD31 and anti-VCAM1 (Abcam, ab19569) antibodies, followed by the secondary A568-conjugated anti–rabbit antibody (Invitrogen, A110011) and the A488-conjugated anti-rat antibody (Invitrogen, A11006), respectively. Mounting medium containing DAPI (Sigma-Aldrich) was used to stain the nuclei and images were acquired using a Leica TCS SP8X White Light Laser confocal system. Quantifications were performed using ImageJ software analysis
Hatzioannou A., Banos A., Sakelaropoulos T., Fedonidis C., Vidali M.S., Köhne M., Händler K., Boon L., Henriques A., Koliaraki V., Georgiadis P., Zoidakis J., Termentzi A., Beyer M., Chavakis T., Boumpas D., Tsirigos A, & Verginis P. (2019). An intrinsic role of IL-33 in Treg cell-mediated tumor immunoevasion. Nature immunology, 21(1), 75-85.
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