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Fixable fluorescent viability stain

Manufactured by Thermo Fisher Scientific
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The Fixable fluorescent viability stain is a cell labeling reagent that selectively stains dead cells, allowing for the identification and quantification of live and dead cells in a sample. It provides a simple and reliable method for assessing cell viability.

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Lab products found in correlation

Influx flow cytometer system, by BD (2 mentions) Triton x 100, by Merck Group (2 mentions) Rnase inhibitor, by Takara Bio (2 mentions) Influx cytometer, by BD (1 mentions) Moflo xdp, by BD (1 mentions)

Spelling variants of this product

Viability stain (9 citations) Fixable viability stain (2 citations)

3 protocols using fixable fluorescent viability stain

1

Single-Cell Isolation and Sorting

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Single cells were isolated from processed tissues using fluorescence-activated cell sorting (FACS). Once processed, samples were incubated with a fixable fluorescent viability stain (Life Technologies) for 20mins (diluted 1:1000 in PBS) prior to incubation with conjugated primary antibodies for 30 mins at 4°C. Antibodies were diluted 1:300 in PBS 0.5% BSA. Stained samples were index sorted, using the BD influx flow cytometer system, Single-cells were sorted into 2 μL of Lysis Buffer (1:20 solution of RNase Inhibitor (Clontech, cat. no. 2313A) in 0.2% v/v Triton X-100 (Sigma-Aldrich, cat. no. T9284)) in 96 well plates, spun down and immediately frozen at −80 degrees.
Davidson S., Efremova M., Riedel A., Mahata B., Pramanik J., Huuhtanen J., Kar G., Vento-Tormo R., Hagai T., Chen X., Haniffa M.A., Shields J.D, & Teichmann S.A. (2020). Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth. Cell Reports, 31(7), 107628.
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2

Single-cell Isolation and Sorting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cells were isolated from processed tissues using fluorescence-activated cell sorting (FACS). Once processed, samples were incubated with a fixable fluorescent viability stain (Life Technologies) for 20mins (diluted 1:1000 in PBS) prior to incubation with conjugated primary antibodies for 30 mins at 4°C. Antibodies were diluted 1:300 in PBS 0.5% BSA. Stained samples were index sorted, using the BD influx flow cytometer system, Single-cells were sorted in 2μl of Lysis Buffer (1:20 solution of RNase Inhibitor (Clontech, cat. no. 2313A) in 0.2% v/v Triton X-100 (Sigma-Aldrich, cat. no. T9284)) in 96 well plates, spun down and immediately frozen at -80 degrees.
Davidson S., Efremova M., Riedel A., Mahata B., Pramanik J., Huuhtanen J., Kar G., Vento-Tormo R., Hagai T., Chen X., Haniffa M.A., Shields J.D, & Teichmann S.A. (2020). Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth. Cell Reports, 31(7), 107628.
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3

Single-Cell Tumor Sample Fixation and Flow Cytometry

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Once processed, single-cell suspension tumor samples were incubated with a fixable fluorescent viability stain (Life Technologies) for 20 mins (diluted 1:1000 in PBS) prior to incubation with conjugated primary antibodies for 30 mins at 4 °C. Antibodies were diluted in PBS with 0.5% bovine serum albumin (BSA). Stained samples were sorted, using the MoFlo XDP or BD Influx cytometer system.
Mahata B., Pramanik J., van der Weyden L., Polanski K., Kar G., Riedel A., Chen X., Fonseca N.A., Kundu K., Campos L.S., Ryder E., Duddy G., Walczak I., Okkenhaug K., Adams D.J., Shields J.D, & Teichmann S.A. (2020). Tumors induce de novo steroid biosynthesis in T cells to evade immunity. Nature Communications, 11, 3588.
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