The Joint Institutional Review Board of Taipei Medical University accorded the authorization for this research (N202201135). At the time of admission, all patients provided written informed consent before undergoing diagnostic and therapeutic procedures.Throughout this study, the Helsinki Declaration’s principles were implemented. At the timeframes and dosages indicated, cells were exposed to human recombinant GDF15 (PeproTech, Rocky Hill, NJ, USA), MAPK14 inhibitor, or mouse antihuman GDF15 monoclonal antibody (R&D Systems, Minneapolis, MN, USA). Long-course treatment with GDF15 includes centrifuging the cells at 300× g twice weekly and resuspending them in CM containing GDF15.
Human recombinant gdf15
Manufactured by Thermo Fisher Scientific
Sourced in United States
Human recombinant GDF15 is a growth differentiation factor that is produced using recombinant DNA technology. It belongs to the transforming growth factor-beta (TGF-β) superfamily of proteins. GDF15 is involved in regulating cellular processes, but its specific functions are still under investigation.
Automatically generated - may contain errors
Lab products found in correlation
Rapamycin, by Merck Group (2 mentions)
Antibiotic antimycotic solution, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Poly l lysine (pll), by Merck Group (2 mentions)
Ly364947, by Merck Group (1 mentions)
U0126, by Merck Group (1 mentions)
Ly2109761, by Merck Group (1 mentions)
Sb431542, by Merck Group (1 mentions)
U0126, by Selleck Chemicals (1 mentions)
4 protocols using human recombinant gdf15
1
Recombinant GDF15 Effects on Cells
2
GDF15 Modulates Adipogenic Process
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During the adipogenic process, cells were treated with human recombinant GDF15 (100ng/mL, Peprotech, Rocky Hill, NJ, USA) or a neutralizing anti-GDF15 antibody (4 μg/mL, R&D, Minneapolis, MN, USA) in induction medium at the indicated doses for the indicated time periods.
3
GDF15 Regulates Kv2.1 Expression
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Recombinant human GDF15 was purchased from Pepro Tech. We tested whether its biological activity was required for the GDF15-induced Kv2.1 expression by using heat-inactivated GDF15. After heat and radiation inactivation, the effect of GDF15 on Kv2.1 expression was diminished (results not shown). TTX, 4-AP, DRB (5,6-dichlorobenzimidazole riboside), CHX (cycloheximide), U0126, rapamycin, LY364947, PP2 and poly-L -lysine were purchased from Sigma. FBS, DMEM and antibiotic/antimycotic solution were purchased from Gibco Life Technologies.
4
GDF-15 Recombinant Protein Synthesis
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Recombinant human GDF-15 was purchased from Pepro Tech. TTX, 4-AP, rapamycin, SB431542, PP1, LY2109761 and poly-L -lysine were purchased from Sigma. U0126 was purchased from Selleckchem. FBS, DMEM and antibiotic/antimycotic solution were purchased from Gibco Life Technologies.
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