1 α pgc1 α

Primary antibodies used were as follows: mouse anti-complex V β subunit (CxVβ; for Western Blotting-WB-, 1:1000; for Immunocytochemistry-ICC-, 1:200; Abcam, Cambridge, UK), rabbit anti-peroxisome proliferator-activated receptor γ co-activator 1 α (PGC1-α; for WB, 1:200; for ICC, 1:200; Santa Cruz Biotechnologies, CA, USA), rabbit anti- mtTFA (TFAM; 1:1000, Abcam Cambridge, UK), mouse anti-Mitofusin1 (Mfn1; 1:1000; Abcam, Cambridge, UK), mouse anti-Mitofusin2 (Mfn2; 1:1000; Abcam, Cambridge, UK) mouse anti-mitochondrial dynamin-like GTPase (OPA1; 1:1000; Novus Biologicals, CO, USA), rabbit anti-Dynamin-related protein 1 (Drp1; 1:1000, Cell Signalling Technology, MA, USA) mouse anti-β actin HRP (1:25,000; Abcam, Cambridge, UK), rabbit anti-p62/SQSTM1 (for WB, 1:20,000; for ICC, 1:200; Abcam, Cambridge, UK), rabbit anti-LC3 (for WB, 1:1000; for ICC, 1:200; Novus Biologicals, CO, USA). Secondary antibodies used for WB were as follows: goat anti-rabbit HRP secondary antibody (1:5000; Thermo Fisher Scientific, MA, US), goat anti-mouse HRP secondary antibody (1:5000; Abcam, Cambridge, UK). Secondary antibodies used for ICC were as follows: anti-rabbit (Alexa Fluor, emission at 488 nm; 1:1000; Thermo Fisher Scientific, MA, US) or anti-mouse (Alexa Fluor, emission at 568 nm; 1:1000; Thermo Fisher Scientific, MA, US).

Primary antibodies used were as follows: mouse anti-complex V β subunit (CxVβ; for Western Blotting-WB-, 1:1000; for Immunocytochemistry-ICC-, 1:200; Abcam, Cambridge, UK), rabbit anti-peroxisome proliferator-activated receptor γ co-activator 1 α (PGC1-α; for WB, 1:200; for ICC, 1:200; Santa Cruz Biotechnologies, CA, USA), rabbit anti- mtTFA (TFAM; 1:1000, Abcam Cambridge, UK), mouse anti-Mitofusin1 (Mfn1; 1:1000; Abcam, Cambridge, UK), mouse anti-Mitofusin2 (Mfn2; 1:1000; Abcam, Cambridge, UK) mouse anti-mitochondrial dynamin-like GTPase (OPA1; 1:1000; Novus Biologicals, CO, USA), rabbit anti-Dynamin-related protein 1 (Drp1; 1:1000, Cell Signalling Technology, MA, USA) mouse anti-β actin HRP (1:25,000; Abcam, Cambridge, UK), rabbit anti-p62/SQSTM1 (for WB, 1:20,000; for ICC, 1:200; Abcam, Cambridge, UK), rabbit anti-LC3 (for WB, 1:1000; for ICC, 1:200; Novus Biologicals, CO, USA). Secondary antibodies used for WB were as follows: goat anti-rabbit HRP secondary antibody (1:5000; Thermo Fisher Scientific, MA, US), goat anti-mouse HRP secondary antibody (1:5000; Abcam, Cambridge, UK). Secondary antibodies used for ICC were as follows: anti-rabbit (Alexa Fluor, emission at 488 nm; 1:1000; Thermo Fisher Scientific, MA, US) or anti-mouse (Alexa Fluor, emission at 568 nm; 1:1000; Thermo Fisher Scientific, MA, US).