Whole Pancreatic cancer cells were lysed on ice in a lysis buffer (RIPA, Beyotime, Shanghai, China) with a protease inhibitor mixture cocktail (Roche, Switzerland) after culturing in MRC-5-CM for 14 days. Western-blot analysis was performed by established protocols 43 (link). Anti-CTNNA1 and CTNNB1, anti-E-cadherin, anti-N-cadherin, anti-ZEB-1, ZEB-2, Snail, Slug and anti-vimentin primary antibodies were purchased from (Abcam); anti-MMP-2, 3, 13 and 21, anti-WNT-2, anti-WNT16, anti-TGFB1, anti-Src, P-Src-Y418 and P-Src-Y529 primary antibodies were purchased from (Epitomics); anti-Bcl-2, Bcl-xl, Bad, Bax, Bim, Bid, Caspase-3 and anti-β-actin were from ( Cell Signaling Technology).
P src y529
Manufactured by Abcam
P-Src-Y529 is an antibody that detects the phosphorylation of tyrosine 529 on the Src protein. Src is a proto-oncogene tyrosine-protein kinase that plays a role in cell growth and division. Phosphorylation of tyrosine 529 on Src is associated with its inactivation.
Automatically generated - may contain errors
Lab products found in correlation
Protease inhibitor mixture cocktail, by Roche (2 mentions)
Anti zeb1, by Abcam (2 mentions)
Anti wnt 2, by Abcam (2 mentions)
Anti wnt16, by Abcam (2 mentions)
Anti src, by Abcam (2 mentions)
P src y418, by Abcam (2 mentions)
Snail, by Abcam (2 mentions)
Anti β actin, by Cell Signaling Technology (2 mentions)
Anti e cadherin, by Abcam (2 mentions)
Radioimmunoprecipitation assay (ripa), by Beyotime (2 mentions)
Anti n cadherin, by Abcam (2 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by Abcam (1 mentions)
Anti tgfb1, by Abcam (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Anti vimentin, by Abcam (1 mentions)
Anti zo 1, by Abcam (1 mentions)
Cyclin a, by Cell Signaling Technology (1 mentions)
Anti fibronectin, by Abcam (1 mentions)
Cyclin e2, by Cell Signaling Technology (1 mentions)
2 protocols using p src y529
1
Molecular Mechanisms of Pancreatic Cancer Progression
2
Molecular Profiling of Epithelial-Mesenchymal Transition
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Experimental cancer cells (after culturing in HUVEC-CM for 21 days) and respective control cells were lysed on ice in a lysis buffer (RIPA, Beyotime, Shanghai, China) with a protease inhibitor mixture cocktail (Roche, Switzerland). Western-blot analysis was performed by established protocols 12 . Anti-α-catenin, anti-E-cadherin, anti-ZEB-1, ZEB-2, Snail, Slug, anti-ZO-1 and anti-Laminin A1 primary antibodies were purchased from (Abcam); anti-MMP-1, -2, -3, -11, -12, -13, -17 and -21, anti- ITGA6, B1, B3, B4, B7, anti-FAK, P-FAK-Y397, anti-Src, P-Src-Y418, P-Src-Y529, anti-fibronectin, anti-Laminin B3, anti-Wnt-2, anti-Wnt-5B, anti-Wnt-16, anti-TGF-β, anti-β-catenin and anti-N-cadherin primary antibodies were purchased from (Epitomics); anti-Cdc-2, P-Cdc-2-Tyr15, CDK4, CDK6, Cyclin A, Cyclin D1, Cyclin D3, Cyclin E2, P15, P16, P21, P27, P53, Rb, P-Rb-S811, P-Rb-S780; anti-Bcl-xl, Bad, P-Bad-Ser112, Bak, Mcl-1, Puma and anti-β-actin were from (Cell Signaling Technology).
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