SDS-PAGE gels were transferred via a semidry blotter to polyvinylidene difluoride transfer membranes and blocked for 4 h with TBS-T containing 10% (wt/vol) milk powder. Primary antibodies were used to detect Hsp90 (rabbit anti-HSP90AB1 [11405-1-AP; Proteintech]), Hsp70 (mouse anti-Hsp70 [ab2787]), CK2a (rabbit anti-CSNK2A1 [ab10466]), NS2 (guinea pig anti-NS2 serum), VP2 (rabbit anti-VP2 serum), VP5 (guinea pig anti-VP5 serum), VP7 (guinea pig anti-VP7 serum), NS1 (rabbit anti-NS1 serum), NS3 (rabbit anti-NS3 serum), GFP (mouse anti-GFP [G1546-200UL]; Sigma-Aldrich Co), and GAPDH (rabbit anti-GAPDH (ab9485; Abcam). These were added to blocked membranes and incubated overnight at 4°C. Secondary antibodies included an alkaline phosphatase-conjugated goat anti-guinea pig immunoglobulin G (1:10,000; Sigma-Aldrich Co., A5062), goat anti-rabbit (A0418), and an alkaline phosphatase-conjugated goat anti-mouse (A3562) IgG (1:10,000; Sigma-Aldrich Co.), respectively.
Alkaline phosphatase conjugated goat anti mouse a3562 igg
Manufactured by Merck Group
Alkaline phosphatase-conjugated goat anti-mouse (A3562) IgG is a laboratory reagent used as a detection tool. It consists of goat-derived antibodies specific to mouse immunoglobulin G (IgG) that are conjugated to the enzyme alkaline phosphatase. This conjugate can be used to detect the presence of mouse IgG in various immunoassay techniques.
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2 protocols using alkaline phosphatase conjugated goat anti mouse a3562 igg
1
Western Blot Analysis of Viral Proteins
2
SDS-PAGE and Western Blot Analysis
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SDS-PAGE gels were transferred via a semidry blotter to PVDF transfer membranes and blocked for 4 h with Tris-buffered saline containing 0.05% Tween 20 and 10% (w/v) milk powder. The following primary antibodies were used for the detection of: NS2 (guinea pig anti-NS2 serum), VP3 (rabbit anti-VP3 serum), β-Actin (monoclonal anti-β-Actin antibody produced in mouse, clone AC-74, A2228, Sigma-Aldrich), GAPDH (rabbit anti-GAPDH (ab9485, Abcam), anti-CKII α antibody (ab10466, Abcam). These were added to blocked membranes and incubated overnight at 4 °C. Secondary antibodies were an alkaline phosphatase-conjugated goat anti-guinea pig IgG (1:10,000, Sigma-Aldrich, A5062), goat anti-rabbit (A0418), and an alkaline phosphatase-conjugated goat anti-mouse (A3562) IgG (1:10,000, Sigma-Aldrich).
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