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Ultrasensitive mouse insulin and glucagon elisa kits

Manufactured by Mercodia
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Sourced in Sweden

The Ultrasensitive mouse insulin and glucagon ELISA kits are laboratory equipment designed for the quantitative determination of insulin and glucagon levels in mouse samples. These kits utilize the enzyme-linked immunosorbent assay (ELISA) technique to provide accurate and sensitive measurements of the target analytes.

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Lab products found in correlation

Accu chek glucometer, by Roche (1 mentions) Aprotinin, by Merck Group (1 mentions)

Close spelling variants of this product

Ultrasensitive Mouse Insulin ELISA kit Ultrasensitive Insulin ELISA kit Ultrasensitive Mouse Insulin Kit Ultrasensitive Mouse Insulin ELISA Mouse glucagon ELISA kit Ultrasensitive mouse ELISA kit Mouse insulin ELISA kit Ultrasensitive Insulin ELISA Glucagon ELISA kit Ultrasensitive ELISA kit

2 protocols using ultrasensitive mouse insulin and glucagon elisa kits

1

Circadian Regulation of Metabolic Hormones

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Mice were sacrificed by decapitation at ZT0, ZT4, ZT8, ZT12, ZT16, and ZT20 with subsequent blood collection and serum preparation by immediate centrifugation at 3000 rpm for 15 min at 4°C (Supplemental Fig. S1) and storage at −80°C. Protease inhibitors PMSF (Axon), aprotinin (Sigma), and DPP4 (Millipore) were added to the samples to preserve the hormones from degradation. Insulin and glucagon concentrations were assessed by ultrasensitive mouse insulin and glucagon ELISA kits (Mercodia), and serum glucose was assessed by Accu-Chek glucometer (Roche).
Petrenko V., Saini C., Giovannoni L., Gobet C., Sage D., Unser M., Heddad Masson M., Gu G., Bosco D., Gachon F., Philippe J, & Dibner C. (2017). Pancreatic α- and β-cellular clocks have distinct molecular properties and impact on islet hormone secretion and gene expression. Genes & Development, 31(4), 383-398.
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2

Measurement of Pancreatic Hormones and Liver Glycogen

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Protein extracts from total pancreas were prepared as described (Strom et al., 2007 (link)). Blood samples were collected in EDTA-coated tubes and plasma was separated by centrifugation. Insulin and glucagon concentrations were measured using Ultrasensitive Mouse Insulin and Glucagon ELISA kits (Mercodia, Sweden), respectively. Glycogen concentration was measured from the supernatatant of homogenized liver tissue using a glycogen asssay kit (Sigma).
Damond N., Thorel F., Moyers J.S., Charron M.J., Vuguin P.M., Powers A.C, & Herrera P.L. (2016). Blockade of glucagon signaling prevents or reverses diabetes onset only if residual β-cells persist. eLife, 5, e13828.
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