Log In Sign up
The largest database of trusted experimental protocols

Ficoll sepmate pbmc isolation tubes

Manufactured by STEMCELL
Visit Supplier
Sourced in Canada

Ficoll (SepMATETM PBMC Isolation Tubes) is a laboratory product designed for the isolation of peripheral blood mononuclear cells (PBMCs) from whole blood samples. The product utilizes a density gradient centrifugation method to separate and enrich the PBMC fraction.

Automatically generated - may contain errors

Lab products found in correlation

Taqman gene expression assay, by Thermo Fisher Scientific (2 mentions) Taqman advanced mirna cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Maxwell rsc mirna tissue kit, by Promega (1 mentions) Maxwell rsc mirna plasma and serum kit, by Promega (1 mentions)

Close spelling variants of this product

SepMate tubes (281 citations) SepMate (68 citations) SepMate PBMC isolation tubes (58 citations) Ficoll (25 citations) PBMCs (17 citations) SepMate isolation tubes (10 citations) SepMate isolation (3 citations) PBMC isolation tubes (2 citations)

2 protocols using ficoll sepmate pbmc isolation tubes

1

Isolation and miRNA analysis of PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
In this work, we used the following reagents Ficoll (SepMATETM PBMC Isolation Tubes, StemCell technologies, Vancouver, BC, Canada), Maxwell RSC miRNA Tissue Kit (Cat.# AS1460, Promega, Madison, WI, USA), TaqMan® Advanced miRNA Assays–single tube assays (Cat.#A25576, Thermo Fisher, Waltham, MA, USA), TaqMan® Advanced miRNA cDNA Synthesis Kit (Cat.# A28007, Thermo Fisher, Waltham, MA, USA). Taqman Gene Expression Assay (Thermo Fisher, Walthman, MA, USA) were used to measure expression of hsa-miR30a-5p (Assay ID: 000417) and has-miR16-3p (Assay ID:002171).
Hueso M., Mallén A., Casas Á., Guiteras J., Sbraga F., Blasco-Lucas A., Lloberas N., Torras J., Cruzado J.M, & Navarro E. (2020). Integrated miRNA/mRNA Counter-Expression Analysis Highlights Oxidative Stress-Related Genes CCR7 and FOXO1 as Blood Markers of Coronary Arterial Disease. International Journal of Molecular Sciences, 21(6), 1943.
+ Open protocol
+ Expand
2

Profiling Immune Markers and miRNAs

Check if the same lab product or an alternative is used in the 5 most similar protocols
In this work, we used the following reagents. Ficoll (SepMATETM PBMC Isolation Tubes, StemCell technologies, Vancouver, BC, Canada), Maxwell RSC miRNA Tissue Kit (Cat. # AS1460, Promega, Madison, WI, USA), Maxwell RSC miRNA Plasma and Serum Kit (Cat. # AS1680, Promega, Madison, WI, USA), TaqMan® Advanced miRNA Assays–single tube assays (Cat. #A25576, ThermoFisher, Waltham, MA, USA), TaqMan® Advanced miRNA cDNA Synthesis Kit (Cat. # A28007, ThermoFisher, Waltham, MA, USA). Furthermore, the following TaqMan Gene Expression Assays (ThermoFisher, Waltman, MA, USA) were used to measure the expression of different genes:

IL1α (Hs_00174092_m1),

IL6 (Hs_00174131_m1),

TNFα(Hs_00174129_m1),

CCR5 (Hs_00152917_m1),

Cx3CR1 (Hs_01922583_m1),

MAPK1 (Hs_01046830_m1),

MAP2K1 (Hs_05512159_s1),

MAPK9 (Hs_01558224_m1)

ACTIN (Hs_01060665-g1)

hsa_miR30a-5p (Assay ID:000417)

hsa-miR16-3p (Assay ID:002171), and

cel-miR-54-3p (Assay ID:001361).

Casas A., Mallén A., Blasco-Lucas A., Sbraga F., Guiteras J., Bolaños N., Castaño E., Torras J., Cruzado J.M., Navarro E, & Hueso M. (2020). Chronic Kidney Disease-Associated Inflammation Increases the Risks of Acute Kidney Injury and Mortality after Cardiac Surgery. International Journal of Molecular Sciences, 21(24), 9689.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!