Gelatin from porcine skin (Type A, SLCC7838), methacrylic anhydride (MA), Irgacure (2‐Hydroxy‐4′‐(2‐hydroxye‐thoxy)‐2‐methylpropiophenone), and PBS were obtained from Sigma‐Aldrich (Wisconsin, U.S.A.). AlamarBlue and qPCR kits were purchased from Bio‐Rad (Hercules, U.S.A). Carbopol ETD 2020 polymer was purchased from Lubrizol (Wickliffe, U.S.A.). Calcein AM and propidium iodide (PI) were obtained from Biotium (Fremont, U.S.A.). HUVEC culture medium (VascuLife VEGF) was purchased from Lifeline Cell Technology (Oceanside, U.S.A.). Conjugated F‐actin and wheat germ agglutinin (WGA) were purchased from ThermoFisher, USA; CD31, Connexin 43, Phox2B and Synaptophysin antibodies were purchased from Abcam, USA.
Qpcr kits
Manufactured by Bio-Rad
Sourced in United States
The QPCR (Quantitative Polymerase Chain Reaction) kits from Bio-Rad are designed for the detection and quantification of nucleic acid sequences. These kits utilize real-time PCR technology to accurately measure the amount of a target DNA or RNA sequence in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Synaptophysin, by Abcam (1 mentions)
Propidium iodide, by Biotium (1 mentions)
Calcein am, by Biotium (1 mentions)
Methacrylic anhydride, by Merck Group (1 mentions)
Connexin 43, by Abcam (1 mentions)
Phox2b, by Abcam (1 mentions)
Type a gelatin from porcine skin, by Merck Group (1 mentions)
Wheat germ agglutinin wga, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Alamarblue, by Bio-Rad (1 mentions)
Rnapure high purity total rna rapid extraction kit, by BioTeke (1 mentions)
2 protocols using qpcr kits
1
Porcine Skin Gelatin Hydrogel Fabrication
2
Quantifying Cancer Cell mRNA Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was extracted from the cancer cells using the RNA pure High-purity Total RNA Rapid Extraction Kit (BioTeke, RP1201, China) following the manufacturer’s protocol. Real-time (RT) and quantitative polymerase chain reaction (qPCR) kits (Bio-Rad) were used to determine the mRNA expression levels. The RT and qPCR reactions were conducted as previously described [18 (link)]. Relative mRNA expression was calculated using the comparative cycle threshold (CT) (2−ΔΔCT) method. GAPDH was used as an endogenous control to normalize the data.
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