Normal human dermal fibroblast (NHDF) (Lonza, Basel, Switzerland) were kept in the incubator at 37 °C and 5% CO2 atmosphere. Cells were grown in fibroblast growth basal medium (FBM, Lonza, Basel, Switzerland) supplemented with insulin, human fibroblast growth factor, and gentamicin sulfate-amphotericin at 0.1% (v/v) each (Lonza, Basel, Switzerland), as recommended by the manufacturer.
Normal human dermal fibroblast
Manufactured by Lonza
Sourced in Switzerland
Normal human dermal fibroblast (NHDF) is a primary cell line derived from normal human skin. NHDF cells are used in various cell culture applications to study cellular processes and functions.
Automatically generated - may contain errors
Lab products found in correlation
Human fibroblast growth factor, by Lonza (2 mentions)
Gentamicin sulfate amphotericin, by Lonza (2 mentions)
Hacat, by Thermo Fisher Scientific (2 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (2 mentions)
Glutamine, by Nacalai Tesque (1 mentions)
Penicillin, by Nacalai Tesque (1 mentions)
Streptomycin, by Nacalai Tesque (1 mentions)
Dulbecco modified eagle medium (dmem), by Nacalai Tesque (1 mentions)
Follicle dermal papilla cell medium, by PromoCell (1 mentions)
Hmvec, by Lonza (1 mentions)
Fgmtm 2 fibroblast growth medium 2, by Lonza (1 mentions)
6 protocols using normal human dermal fibroblast
1
Culture of Normal Dermal Fibroblasts
2
Normal Human Dermal Fibroblast Culture
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Normal human dermal fibroblast (NHDF) (Lonza, Basel, Switzerland) was kept in the incubator at 37 °C and 5% CO2 atmosphere. Cells were grown in fibroblast growth basal medium (FBM, Lonza, Basel, Switzerland) supplemented with insulin, human fibroblast GF, and gentamicin sulfate–amphotericin at 0.1% (v/v) each (Lonza, Basel, Switzerland), as recommended by the manufacturer.
3
Culturing Normal Human Dermal Fibroblasts
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Normal human dermal fibroblast (NHDF) (Lonza, Basel, Switzerland) were kept in the incubator at 37°C and 5% CO2 atmosphere. Cells were grown in fibroblast growth basal medium (FBM, Lonza, Basel, Switzerland) supplemented with insulin, human fibroblast growth factor, and gentamicin sulphate-amphotericin at 0.1% (v/v) each (Lonza, Basel, Switzerland), as recommended by the manufacturer.
4
Culturing Normal Human Dermal Fibroblasts
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Normal human dermal fibroblast (NHDF) was purchased from Lonza Co. Ltd. (Tokyo, Japan). Fibroblasts were grown in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque, Kyoto, Japan) supplemented with 2% fetal bovine serum (FBS; MP Biomedicals, Illkirch, France), 2 mM glutamine, 100 units/ml penicillin and 100 mg/ml streptomycin (Nacalai Tesque). The cells were incubated under 37°C in a humidified atmosphere of 5% CO2 and 95% air in a CO2 incubator. After 24 h, culture medium was replaced by the DMEM containing 2% FBS and P40, and the cells were subsequently pre-cultured for 1 week in the CO2 incubator until the experiments.
5
Isolation and Characterization of Human Skin Cell Lines
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• Human hair follicle dermal papilla cell line (FDPC) was obtained from PromoCell (Germany; lot 2040301.35). FDPC were grown in Follicle Dermal Papilla Cell Medium (PromoCell) with 1% antibiotic/antimycotic (Invitrogen, Grand Island, NY, USA). In order to avoid phenotypical changes occurring during cell culture, only FDPC at 2-7 passages were used for all experiments. To confirm FDPC stability we measured alkaline phosphatase activity (ALP). ALP activity: (U/ml)= A/V/T, 1.62 (Bassino et al., 2015) .
• Human Adult Keratinocyte cell line (HaCaT) was obtained from Cell Line Services (CLS Cell Lines Service, Germany). HaCaT were grown in Dulbecco's Modified Eagle medium (DMEM) with 10% FCS and 1% antibiotic/antimycotic (Invitrogen, Grand Island, NY, USA).
• Human microvascular endothelial cell line (HMVEC) was purchased from Lonza (Human adult dermal microvascular endothelial cells, include cells isolated from small vessels within skin tissue from either adults or neonatal foreskins) and grown in Endothelial Cell Grown Medium (EGM 2-MV medium, Lonza).
• Normal Human Dermal Fibroblast (NHDF) was purchased from Lonza and grown in FGM™-2 Fibroblast Growth Medium-2 (Lonza) that contains 2% serum and 1% antibiotic/antimycotic (Invitrogen, Grand Island, NY, USA).
All cell types were maintained at 37°C in a humidified atmosphere of 5% CO 2 .
• Human Adult Keratinocyte cell line (HaCaT) was obtained from Cell Line Services (CLS Cell Lines Service, Germany). HaCaT were grown in Dulbecco's Modified Eagle medium (DMEM) with 10% FCS and 1% antibiotic/antimycotic (Invitrogen, Grand Island, NY, USA).
• Human microvascular endothelial cell line (HMVEC) was purchased from Lonza (Human adult dermal microvascular endothelial cells, include cells isolated from small vessels within skin tissue from either adults or neonatal foreskins) and grown in Endothelial Cell Grown Medium (EGM 2-MV medium, Lonza).
• Normal Human Dermal Fibroblast (NHDF) was purchased from Lonza and grown in FGM™-2 Fibroblast Growth Medium-2 (Lonza) that contains 2% serum and 1% antibiotic/antimycotic (Invitrogen, Grand Island, NY, USA).
All cell types were maintained at 37°C in a humidified atmosphere of 5% CO 2 .
6
Cultivation of Human Skin Cell Lines
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Human Adult Keratinocyte cell line (HaCaT) was obtained from Cell Line Services (CLS Cell Lines Service, Germany). HaCaT were grown in Dulbecco's Modified Eagle medium (DMEM) with 10% fetal calf serum (FCS) and 1% antibiotic/antimycotic (Invitrogen, Grand Island, NY).
Human microvascular endothelial cell line (HMVEC) was purchased from Lonza and grown in Endothelial Cell Grown Medium (EGM 2-MV medium, Lonza, Basel, Switzerland).
Normal human dermal fibroblast (NHDF) was purchased from Lonza and grown in FGMTM-2 Fibroblast Growth Medium-2 (Lonza) that contains 2% serum and 1% antibiotic/antimycotic (Invitrogen, Grand Island, NY).
All cell types were maintained at 37 °C in a humidified atmosphere of 5% CO2.
Human microvascular endothelial cell line (HMVEC) was purchased from Lonza and grown in Endothelial Cell Grown Medium (EGM 2-MV medium, Lonza, Basel, Switzerland).
Normal human dermal fibroblast (NHDF) was purchased from Lonza and grown in FGMTM-2 Fibroblast Growth Medium-2 (Lonza) that contains 2% serum and 1% antibiotic/antimycotic (Invitrogen, Grand Island, NY).
All cell types were maintained at 37 °C in a humidified atmosphere of 5% CO2.
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