Luminex magpix system rcytomag 80 k

Manufactured by Merck Group

The Luminex/MAGPIX system is a multiplex assay platform that utilizes color-coded magnetic beads to simultaneously detect and quantify multiple analytes in a single sample. The system employs flow cytometry technology to rapidly analyze the contents of the beads. This equipment enables the simultaneous measurement of up to 80 different analytes in a single well.

Automatically generated - may contain errors

Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (4 mentions) Mammalian protein extraction reagent, by Thermo Fisher Scientific (2 mentions) Complete tablet, by Merck Group (2 mentions)

Close spelling variants of this product

Luminex MAGPIX system Luminex MAGPIX MAGPIX system Luminex system MAGPIX

2 protocols using luminex magpix system rcytomag 80 k

Quantifying Inflammatory Cytokines in Tissue Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissues were homogenized in Mammalian Protein Extraction Reagent® (Thermo Fisher Scientific, Waltham, MA) and a protease inhibitor cocktail (cOmplete® tablets, Sigma-Aldrich, St. Louis, MO), with a POLYTRON homogenizer, followed by 10 minutes disruption in a bead beater and sonication for 1 min. Samples were centrifuged at 13000 rpm for 5 min, and total protein concentration was quantified using the BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). Samples were diluted as needed in order to standardize the amount of protein. Granulocyte-macrophage colony-stimulating factor (GM-CSF), Interleukin 1 alpha (IL-1α), IL-1β, IL-6, IL-10, keratinocyte chemoattractant (KC) and Tumor Necrosis Factor-alpha (TNFα) were simultaneously quantified in each sample using the Luminex/MAGPIX system (RCYTOMAG-80 K; Millipore, Billerica, MA).

Bertolini M., Sobue T., Thompson A, & Dongari-Bagtzoglou A. (2017). Chemotherapy Induces Oral Mucositis in Mice Without Additional Noxious Stimuli. Translational Oncology, 10(4), 612-620.

+ Open protocol

+ Expand

Quantification of Inflammatory Mediators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissues were homogenized in Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) and a protease inhibitor cocktail (cOmplete tablets, Sigma-Aldrich), with a POLYTRON homogenizer, followed by 10 minutes disruption in a bead beater and sonication for 1 min. Samples were centrifuged at 13000 rpm for 5 min, and total protein concentration was quantified using the BCA protein assay kit (Thermo Fisher Scientific). Samples were diluted as needed in order to standardize the amount of protein. IL-6, keratinocyte chemoattractant (KC) and Tumor Necrosis Factor-alpha (TNFα) were simultaneously quantified in each sample using the Luminex/MAGPIX system (RCYTOMAG-80K; Millipore).

Bertolini M., Ranjan A., Thompson A., Diaz P.I., Sobue T., Maas K, & Dongari-Bagtzoglou A. (2019). Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection. PLoS Pathogens, 15(4), e1007717.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!