/ce:inf>-cortisol, D4-cortisol) and deuterated cortisone (1,2-[2H]2-cortisone, D2-cortisone) were supplied by Cambridge Isotopes (Tewksbury, MA, USA). Mifepristone (RU486), canrenone, alfaxolone and deuterated canrenone (2,2,4,7,22,22-[2H]6-canrenone, D6-canrenone) were supplied by Sigma Aldrich (St. Louis, MO, USA). Each of these steroids were weighed and dissolved in methanol (final concentration 1 mg/mL) and stored at −20 °C.
Canrenone
Manufactured by Merck Group
Sourced in United States
Canrenone is a laboratory product manufactured by Merck Group. It is a chemical compound used in research and analysis applications. Canrenone serves as a key intermediate in the synthesis of various pharmaceutical and biological molecules. Its core function is to facilitate chemical reactions and transformations in controlled laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Mifepristone ru486, by Merck Group (2 mentions)
Spironolactone, by Merck Group (2 mentions)
N propylbenzene, by Merck Group (1 mentions)
Uhplc system, by Shimadzu (1 mentions)
Ethanol, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
D4 cortisol, by Cambridge Isotopes (1 mentions)
D2 cortisone, by Cambridge Isotopes (1 mentions)
Aldosterone, by Merck Group (1 mentions)
Norethindrone, by Merck Group (1 mentions)
Fluticasone propionate, by Merck Group (1 mentions)
Tibolone, by Merck Group (1 mentions)
Methyltrienolone r1881, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Luciferin, by Promega (1 mentions)
Nifedipine, by Merck Group (1 mentions)
Temozolomide, by Merck Group (1 mentions)
Fura 2 am, by Thermo Fisher Scientific (1 mentions)
Xestospongin c, by Merck Group (1 mentions)
3 3 diaminobenzidine (dab), by Merck Group (1 mentions)
5 protocols using canrenone
1
Steroid Standard Solution Preparation
2
Quantification of Herbal Powder Formula
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A reference standard of spironolactone was purchased from Research Biochemicals International. Canrenone (purity ≥97%) and the internal standard n-propylbenzene were obtained from Sigma-Aldrich Inc. All chemicals were of analytical grade, including formic acid (98–100%) and ethanol, which were purchased from E. Merck. The pharmaceutical herbal powder formula Yin-Chen-Hao-Tang was purchased from Kaiser Pharmaceutical Co., Ltd. and was quantified by a Shimadzu UHPLC system coupled to an electrospray ionization (ESI) source equipped with an LCMS-8030 triple quadrupole mass spectrometer to guarantee the consistent quality of the herbal formula powder [33 ].
3
Steroid Receptor Activation Assay
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Cell culture materials are from Life Technologies (Cergy-Pontoise, France). Luciferin (sodium salt) was purchased from Promega (Charbonnières, France). Chemical substances used in this study are presented in Table 1
Stock solutions of chemicals were prepared in dimethyl sulfoxide (DMSO) and stored at -20°C. Fresh solution of test chemicals in test medium were prepared before each experiment. The final DMSO concentrations during treatment did not exceed 0.1% (v/v) of the test medium.
Stock solutions of chemicals were prepared in dimethyl sulfoxide (DMSO) and stored at -20°C. Fresh solution of test chemicals in test medium were prepared before each experiment. The final DMSO concentrations during treatment did not exceed 0.1% (v/v) of the test medium.
4
Chemical Reagents for Cell Signaling
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Chemicals used in the preparation of media, and most other chemicals, including DAB (1,4-dideoxy-1,4-imino-D-arabinitol hydrochloride), cAMP (cyclic adenosine monophosphate), temozolomide, xestospongin C (1R,4aR,11R,12aS,13S,16aS,23R,24aS)-eicocahydro-5H,17H-1,23:11,13-diethano-2H,4H-[1,11], dioxacycloeicosino [2,3-b:12,13-b1] dipyridine, nifedipine, and canrenone were purchased from Sigma (St. Louis, MO, United States). TRPC1 channel antibody (SC-15055) was obtained from Santa Cruz Biotechnology (sc-15055 from Santa Cruz, CA, United States). OligofectamineTM Reagent for RNA interference, Opti-MEMI, Ryanodine, and U0126 [1,4-diamino-2,3-dicyano-1,4-bis (2-aminophenylthio) butadiene] were purchased from Calbiochem (La Jolla, CA, United States). Fura-2 AM was obtained from Invitrogen (Carlsbad, CA, United States).
5
Quantitative LC-MS Analysis of Spironolactone
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Spironolactone and canrenone were purchased from Sigma Aldrich (St. Louis, MO, USA), 7-alpha-methylthioSpironolactone from PharmaSynth (Tartu, Estonia), and Spironolactone-D3 (used as an internal standard, IS) from TLC Pharmaceutical Standards (Aurora, Ontario, Canada). LC-MS-grade methanol, tetrahydrofuran (THF), NH4F, and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) were obtained from Sigma Aldrich (St. Louis, MO, USA). Water was purified (18.2 MΩ·cm at 25 °C and a TOC value in the range 2–5 ppb) in house using a Millipore Advantage A10 system from Millipore (Bedford, USA).
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