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2 protocols using fertaric acid

1

Characterization of Phenolic Compounds

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Acetonitrile and methanol were purchased from Thermo Fisher Scientific (France). A Millipore Milli-Q system (Merck Millipore, Molsheim, France) provided ultrapure water. L-leucine, L-isoleucine, L-phenylalanine, L-tyrosine, L-tryptophan, L-proline, citric acid, E-resveratrol, E-piceatannol, catechin, epicatechin, coutaric acid, fertaric acid, E-piceid, kaempferol 3-O-glucoside, and kaempferol 3-O-glucuronide were obtained from Sigma-Aldrich (St. Louis, MO, USA). Caftaric acid was purchased from Carbosynth (Compton, Berkshire, UK). Rhamnetin, E-ε-viniferin, kaempferol 3-O-rutinoside, quercetin 3-O-glucoside, quercetin 3-O-glucuronide, myricetin 3-O-glucoside, procyanidin B1, procyanidin B2, procyanidin B3, procyanidin C1, and kaempferol 3-O-rutinoside were purchased from Extrasynthèse (Genay, France). Pallidol and E-δ-viniferin were graciously provided by Dr. Mary-Lorène Goddard (EA-3991, Université de Haute-Alsace, Colmar, France). E-miyabenol C was obtained from Ambinter (Paris, France).
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2

Quantification of Phenolic Compounds

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Pure phenolic compound standards (caffeic acid, caftaric acid, catechin, coutaric acid, ellagic acid, epicatechin, ethyl gallate, fertaric acid, ferulic acid, gallic acid, kaempferol, kaempferol-3-O-glucoside, kaempferol-3-O-rutinoside, myricetin, myricetin-3-O-rhamnoside, p-coumaric acid, p-OH-benzoic acid, procyanidin B1, procyanidin B2, protocatechuic acid, quercetin, quercetin-3-O-rutinoside, salicylic acid, syringic acid, t-resveratrol, t-piceid and vallinic acid) were purchased from Sigma-Aldrich Chemical Co. (St.
Louis, MO, USA). Anthocyanin standards (cyanidin chloride, delphinidin chloride, malvidin chloride, pelargonidin chloride, peonidin chloride and petunidin chloride) and pelargonidin-3-O-glucoside were obtained from Extrasynthese (Genay, France). Unless otherwise stated, all other chemicals and reagents were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA), Panreac (Barcelona, Spain) or Fisher Scientific (Loughborough, UK).
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