The HPLC system consists of: Elite LaChrom with an L2200 autosampler, L2130 pump, L2350 column oven, L2444 DAD, and EZChrom Elite 3.1.7 software (Hitachi, Tokyo, Japan); column: Kinetex® Biphenyl, 100 Å, 250 × 4.6 mm 5 µm (same material pre-column; Phenomenex, Torrance, CA, USA); injection volume 20 µL; oven temp. 25 °C; auto sampler temp.: 10 °C; detection wavelength: 340 nm; flow: 1.2 mL/min; A = H2O + 0.1% TFA, B = acetonitrile + 0.1% TFA; gradient: 0–2 min 10% B, 2–15 min 10% B → 70% B, 15–16 min 70% B → 10% B, 16–20 min 10% B. All samples were filtered (Phenex RC Membrane 0.2 um, Phenomenex, USA) before injection.
L2350 column oven
Manufactured by Hitachi
Sourced in Japan
The L2350 column oven is a laboratory equipment designed for temperature control and regulation of chromatographic columns. It maintains a stable temperature environment to ensure consistent and reliable chromatographic separations.
Automatically generated - may contain errors
2 protocols using l2350 column oven
1
HPLC Separation and Quantification Protocol
2
Biphenyl HPLC Protocol for Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The system consisted of the following: Elite LaChrom with an L2200 autosampler, L2130 pump, L2350 column oven, L2444 DAD, and EZChrom Elite 3.1.7 software (Hitachi, Tokyo, Japan); column: Kinetex® Biphenyl, 100 Å, 250-4.6 mm (5 µm, same material precolumn; Phenomenex, Torrance, CA, USA); injection volume 30 µL; oven temp.: 25 °C; autosampler temp.: 10 °C; detection wavelength: 340 nm; flow: 1.2 mL/min; A = H2O + 0.1% TFA, B = acetonitrile + 0.1% TFA; gradient: 0–2 min 10% B, 2–10 min 10% B → 100% B, 10–11 min 100% B → 10% B, 11–15 min 10% B.
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