Normal human cardiac fibroblasts (HCFs) were purchased from Cell Applications, Inc (San Diego, CA). Fibroblasts were maintained in low glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 units ml penicillin, and 100 μg/ml streptomycin (Life Technologies, Carlsbad, CA).
Normal human cardiac fibroblasts hcfs
Manufactured by Cell Applications
Normal human cardiac fibroblasts (HCFs) are primary cells derived from the cardiac tissue of healthy human donors. These cells play a crucial role in the maintenance and repair of the heart's extracellular matrix, which provides structural support and mechanical properties to the cardiac tissue.
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Lab products found in correlation
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Penicillin streptomycin, by Beyotime (1 mentions)
Glutamax, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Beyotime (1 mentions)
Bm hmscs, by American Type Culture Collection (1 mentions)
2 protocols using normal human cardiac fibroblasts hcfs
1
Culturing Normal Human Cardiac Fibroblasts
2
Cardiac Fibroblast and Mesenchymal Stem Cell Cultivation
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Normal human cardiac fibroblasts (HCFs) were purchased from Cell Applications, Inc. Fibroblasts were maintained in low glucose Dulbecco's modified Eagle's media (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin (Beyotime Institute of Biotechnology), and 100 µg/ml streptomycin (Life Technologies; Thermo Fisher Scientific, Inc.).
Bone marrow-derived human MSCs (BM-hMSCs) were purchased from ATCC, and seeded at 5,000 cells/cm2 in DMEM-F12 growth media with 1% Glutamax (Sigma-Aldrich; Merck KGaA) and 1% penicillin-streptomycin, and were grown to 80–85% confluency.
Bone marrow-derived human MSCs (BM-hMSCs) were purchased from ATCC, and seeded at 5,000 cells/cm2 in DMEM-F12 growth media with 1% Glutamax (Sigma-Aldrich; Merck KGaA) and 1% penicillin-streptomycin, and were grown to 80–85% confluency.
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