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1 1 diphenyl 2 picryl hydrazyl free radical dpph

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1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) is a stable free radical compound used as a lab reagent. It is commonly used in antioxidant assays to measure the free radical scavenging ability of various substances.

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Lab products found in correlation

Folin ciocalteu s reagent, by Merck Group (2 mentions) Quercetin 3 o glucoside, by Merck Group (2 mentions) Methanol, by Avantor (2 mentions) Formic acid, by Avantor (2 mentions) Hplc grade water, by Avantor (1 mentions) Chlorogenic acid, by Merck Group (1 mentions) Ferulic acid, by Merck Group (1 mentions) Protocatechuic acid, by Merck Group (1 mentions) Vanillic acid, by Merck Group (1 mentions) Syringic acid, by Merck Group (1 mentions) Gallic acid, by Merck Group (1 mentions) Digestive enzymes, by Merck Group (1 mentions) Anthocyanin standards, by Extrasynthese (1 mentions) Folin ciocalteau reagent, by Merck Group (1 mentions) Ethanol, by Avantor (1 mentions) Acetone, by Avantor (1 mentions) Toluene, by Avantor (1 mentions) Trolox, by Merck Group (1 mentions) Potassium persulfate, by Merck Group (1 mentions) 2 2 azinobis 3 ethylbenzthiazoline 6 sulfonic acid, by Merck Group (1 mentions)

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DPPH radical (43 citations) DPPH free radical (9 citations)

5 protocols using 1 1 diphenyl 2 picryl hydrazyl free radical dpph

1

Phytochemical Characterization of Cereal Grains

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Methanol, ethanol, HPLC-grade water, acetonitrile, acetone, toluene, reagents for electrophoretic analysis, and acids were from VWR International (Fontenay-sous-Bois, France). Gliadin, digestive enzymes, Folin-Ciocalteu’s reagent, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH), salts, phenolic acids (ferulic acid, chlorogenic acid, vanillic acid, gallic acid, syringic acid, protocatechuic acid), rutin, and quercetin-3-O-glucoside were purchased from Sigma Aldrich (Steinheim, Germany). Anthocyanin standards were from Extrasynthese (Genay, France). Prestained molecular weight marker solution was from Bio-Rad (Hercules, CA, USA).
Piazza S., Colombo F., Bani C., Fumagalli M., Vincentini O., Sangiovanni E., Martinelli G., Biella S., Silano M., Restani P., Dell’Agli M, & Di Lorenzo C. (2022). Evaluation of the Potential Anti-Inflammatory Activity of Black Rice in the Framework of Celiac Disease. Foods, 12(1), 63.
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2

Antioxidant Capacity Determination Protocol

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Folin-Ciocalteau reagent, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH), trolox, potassium persulfate, 2-2′-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), salts, enzymes, and standards of phenolic acids were purchased from Sigma Aldrich (Steinheim, Germany); ethanol, methanol, acetone, toluene, and formic acid were from VWR International (Fontenay-sous-Bois, France); standards of anthocyanins were from Extrasynthese (Genay, France).
Colombo F., Di Lorenzo C., Petroni K., Silano M., Pilu R., Falletta E., Biella S, & Restani P. (2021). Pigmented Corn Varieties as Functional Ingredients for Gluten-Free Products. Foods, 10(8), 1770.
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3

Quantitative Polyphenol Analysis

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Quercetin 3-O-rutinoside (rutin), quercetin 3-O-galactoside, quercetin 3-O-glucoside, quercetin 3-O-arabinoside, quercetin 3-O-rhamnoside, kaempferol 3-O-rhamnoside and gallic acid (GA) standards were obtained from Yuanye (Shanghai, China). 2,4,6-Tripyridyl-S-triazine (TPTZ), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS·+) and 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH·) were purchased from Sigma-Aldrich (St. Louis, USA). HPLC-grade acetonitrile and methanol were acquired from Alltech Scientific (Beijing, China). Ultrapure water was produced using a Milli-Q System from Millipore (Billerica, USA). All other regents were analytical grade and were obtained from Beijing Chemical Works (Beijing, China).
Su S., Wang L., Geng Y, & Wang J. (2024). Flavonol profiles of mature leaves allow discriminating Toona sinensis Roem from different north-south geographical origins across China with varied antioxidant activities. Heliyon, 10(5), e27040.
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4

Antioxidant Potential of Stingless Bee Cerumen

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The cerumen of Tetragonula laeviceps was purchased from the meliponiculturists in Ramphan village, Tha Mai District, Chanthaburi 22170, Thailand, and shipped to the Singapore Institute of Technology, Singapore. Gum Arabic Grade 1 (Hashab Acacia Senegal) was acquired from Ingredion Pte Ltd., Singapore, and Maltodextrin DE 10–20 was purchased from Suntop Enterprise Pte Ltd., Singapore. Folin–Ciocalteu’s phenol reagent, 1,1-Diphenyl-2-picrylhydrazyl Free Radical (DPPH), gallic acid monohydrate ACS reagent, sodium carbonate anhydrous, and analytical grade ethanol (99.9%) were obtained from Sigma-Aldrich, Singapore.
Iesa N.B., Chaipoot S., Phongphisutthinant R., Wiriyacharee P., Lim B.G., Sringarm K., Burgett M, & Chuttong B. (2023). Effects of Maltodextrin and Gum Arabic Composition on the Physical and Antioxidant Activities of Dewaxed Stingless Bee Cerumen. Foods, 12(20), 3740.
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5

Comprehensive Analytical Standards Protocol

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Acetonitrile, methanol, formic acid and water (LC-MS grade) were purchased from VWR International (Fontenay-sous-Bois, France). Folin-Ciocalteu's reagent, sodium carbonate and reagents used for biological assays were from Sigma Aldrich (Germany). The commercial standards at disposal were: flavonols (quercetin, quercetin-3-O-rutinoside, quercetin-3-Ogalactoside, quercetin-3-O-glucoside, quercetin-3-Oglucuronide, kaempferol and kaempferol-3-O-glucoside), flavan-3-ols (epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, procyanidin B2), the stilbene resveratrol and trans-piceid; these standard as well as the reactive 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) were from Sigma-Aldrich (Steinem, Germany). Organic acids (caftaric acid, ferulic acid, chlorogenic acid and caffeic acid) and anthocyanins (delphinidin-3-O-glucoside, cyanidin-3-Oglucoside, petunidin-3-O-glucoside, peonidin-3-O-glucoside and malvidin-3-O-glucoside) were from Extrasynthèse (Lyon, France). All standards were stored according to the supplier's instructions and then solubilized at concentration of 1 mg/mL in a water:methanol mixture (1:1, v/v), and diluted to a final concentration of 10 μg/mL and injected in the chromatographic system.
Colombo F ., Di Lorenzo C ., Regazzoni L ., Fumagalli M ., Sangiovanni E ., Peres de Sousa L ., Bavaresco L ., Tomasi D ., Bosso A ., Aldini G ., Restani P , & Dell'Agli M . (2019). Phenolic profiles and anti-inflammatory activities of sixteen table grape (Vitis vinifera L.) varieties. Food & function, 10(4).
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