Paraffin-embedded and formalin-fixed HCC samples were cut into 5-μm sections, which were then processed for immunohistochemistry as previously described. Briefly, the sections were incubated with Abs against the following human antigens: CD8 (rabbit monoclonal, clone EP334, ZSBio, China), CD11b (rabbit monoclonal, clone EPR1344, Abcam, UK), CD68 (mouse monoclonal; clone PG-M1; Dako Cytomation, USA), CD15 (mouse monoclonal; clone LeuM1; ZSBio), CD206 (mouse monoclonal, clone 685645, R&D Systems, USA), CD204 (mouse monoclonal, clone SRA-C6, Transgenic, Japan), CD163 (mouse monoclonal, clone 10D6, ZSBio) and CD169 (sheep polyclonal, clone NS0, R&D Systems). Then, the sections were stained in an EnVision System (Dako Cytomation, USA). Positive cells were detected by microscopy and quantified using the Vectra-Inform image analysis system (Perkin-Elmer Applied Biosystems, Foster City, CA, USA).
Ep334
Manufactured by ZSGB-BIO
Sourced in China
The EP334 is a laboratory equipment designed for general scientific applications. It serves as a device for performing essential tasks within a controlled laboratory environment. The core function of the EP334 is to provide a mechanism for controlled and precise measurements or operations, as required by various scientific procedures.
Automatically generated - may contain errors
Lab products found in correlation
Envision system, by Agilent Technologies (2 mentions)
Epr1344, by Abcam (2 mentions)
Pg m1, by Agilent Technologies (2 mentions)
Cd206, by R&D Systems (2 mentions)
Leum1, by ZSGB-BIO (2 mentions)
Cd163, by ZSGB-BIO (2 mentions)
Cd169, by R&D Systems (2 mentions)
Vectra inform image analysis system, by Thermo Fisher Scientific (2 mentions)
Cd11b, by Abcam (2 mentions)
2 protocols using ep334
1
Immunohistochemical Analysis of Immune Cells in HCC
2
Immunohistochemical Analysis of Immune Cells in HCC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin-embedded and formalin-fixed HCC samples were cut into 5-μm sections, which were then processed for immunohistochemistry as previously described. Briefly, the sections were incubated with Abs against the following human antigens: CD8 (rabbit monoclonal, clone EP334, ZSBio, China), CD11b (rabbit monoclonal, clone EPR1344, Abcam, UK), CD68 (mouse monoclonal; clone PG-M1; Dako Cytomation, USA), CD15 (mouse monoclonal; clone LeuM1; ZSBio), CD206 (mouse monoclonal, clone 685645, R&D Systems, USA), CD204 (mouse monoclonal, clone SRA-C6, Transgenic, Japan), CD163 (mouse monoclonal, clone 10D6, ZSBio) and CD169 (sheep polyclonal, clone NS0, R&D Systems). Then, the sections were stained in an EnVision System (Dako Cytomation, USA). Positive cells were detected by microscopy and quantified using the Vectra-Inform image analysis system (Perkin-Elmer Applied Biosystems, Foster City, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!