FreeStyleTM 293F (HEK-293F), Expi293FTM cells (Expi) and FreeStyleTM CHO-S (CHO-S) cells were cultured in FreeStyleTM 293 expression medium and FreeStyleTM CHO expression medium, respectively (Invitrogen). Additional cell lines were obtained from the American Type Culture Collection (ATCC). B16-F10 (C57Bl/6-derived mouse skin melanoma) cells were cultured in DMEM medium containing Ultraglutamin 1 (Lonza), supplemented with 10% (v/v) Donor Bovine Serum with Iron (Life Technologies) and Pen/Strep (Lonza). Raji (human CD20-positive Burkitt’s lymphoma) cells and Daudi (human CD20-positive Burkitt’s lymphoma) cells were cultured in RPMI 1640 medium (Lonza), supplemented with 10% (v/v) Donor Bovine Serum with Iron, 1% (w/v) L-glutamine 200 mM in 0.85% (w/v) NaCl solution (Lonza), 1% Sodium Pyruvate (Lonza) and Pen/Strep (Lonza). All cell lines were maintained at 37 °C in a 5% CO2 humidified incubator.
Nacl solution
Manufactured by Lonza
The NaCl solution is a laboratory-grade product that provides a standardized sodium chloride (NaCl) solution. It serves as a basic saline solution for various experimental and analytical applications in the life sciences and chemical research fields.
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Lab products found in correlation
L glutamine, by Lonza (2 mentions)
Freestyle 293 f, by Thermo Fisher Scientific (1 mentions)
Expi293f cells, by Thermo Fisher Scientific (1 mentions)
Donor bovine serum with iron, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Lonza (1 mentions)
Pen strep, by Lonza (1 mentions)
Hank s balanced salt solution, by Lonza (1 mentions)
2 protocols using nacl solution
1
Culturing of Mammalian Cell Lines
2
BHK-21 Cell Culture and Virus Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Baby hamster kidney (BHK-21;) and BSR cell monolayers [47, 48] were cultured in 75 cm 2 flasks using BioWhittaker Dulbecco's Modified Eagle's Medium (DMEM) (Lonza), supplemented with 5% (v/v) foetal bovine serum (FBS) (HyClone), 1% (v/v) L-glutamine [200 mM in a 0.85% (w/v) NaCl solution, Lonza] and 1% (v/v) PSA (10 000 U/ml of penicillin, 10 000 µg/ml of streptomycin and 25 µg/ml of amphotericin B; HyClone). The flasks were incubated at 37°C, with 5% CO2, in a humidified incubator.
Cell culture inoculations were prepared by resuspending lyophilized preparations of different strains (Table 1) in Hanks balanced salt solution (Lonza) and infecting BHK-21 monolayers at a confluency of 70-80% with the virus. Monolayers were incubated for 3-4 days, or until cytopathic effects were observed. Infected cell monolayers were used for infections and RNA extraction.
Cell culture inoculations were prepared by resuspending lyophilized preparations of different strains (Table 1) in Hanks balanced salt solution (Lonza) and infecting BHK-21 monolayers at a confluency of 70-80% with the virus. Monolayers were incubated for 3-4 days, or until cytopathic effects were observed. Infected cell monolayers were used for infections and RNA extraction.
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