PANC-1 [American Type Culture Collection (ATCC), CRL-1469], BxPC-3 (ATCC, CRL-1687), SK-PC-3, and GP-9A cells were cultured in RPMI 1640 (Sigma-Aldrich). Capan-2 (ATCC, HTB-80) cells were cultured in McCoy 5A (Sigma-Aldrich). MIA PaCa-2 (ATCC, CRL-11268), GP-2A, GP-3A, GP-5A, GP-9A, GP-10A, and GP-16A cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich). All media were supplemented with 2 to 10% fetal bovine serum (Gibco) and cultured under conditions of 5% CO2 in air at 37°C. For primary pancreatic cancer cells, 1× penicillin/streptomycin was added in the growth medium. For hypoxia, cells were transferred to H45 Hypoxystation (Don Whitley Scientific). The DNA-PK inhibitor NU7441 (10 μM, Medchem Express) was added at the time of seeding and replenished 1 hour before collecting the cells.
H45 hypoxystation
Manufactured by Don Whitley Scientific
The H45 Hypoxystation is a controlled atmosphere workstation that maintains a precisely regulated oxygen and carbon dioxide environment. It allows for the simulation and study of specific oxygen and carbon dioxide conditions.
Automatically generated - may contain errors
Lab products found in correlation
Mia paca 2, by American Type Culture Collection (2 mentions)
Bxpc 3, by American Type Culture Collection (2 mentions)
Rpmi 1640, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Panc 1, by American Type Culture Collection (2 mentions)
Capan 2, by American Type Culture Collection (2 mentions)
Mccoy 5a, by Merck Group (2 mentions)
Nu7441, by MedChemExpress (2 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
2 protocols using h45 hypoxystation
1
Culturing Pancreatic Cancer Cell Lines
2
Pancreatic Cancer Cell Culture Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PANC-1 (ATCC, CRL-1469), BxPC-3 (ATCC, CRL-1687), SK-PC-3 and GP-9A cells were cultured in RPMI-1640 (Sigma). Capan-2 (ATCC, HTB-80) cells were cultured in McCoy 5A (Sigma). MIA PaCa-2 (ATCC, CRL-11268), GP-2A, GP-3A, GP-5A, GP-9A, GP-10A and GP-16A cells were cultured in DMEM (Sigma). All media was supplemented with 2%-10% fetal bovine serum (Gibco) and cultured under conditions of 5% CO2 in air at 37⁰C. For primary pancreatic cancer cells 1X penicillin/streptomycin was added in the growth media. For hypoxia, cells were transferred to a H45 Hypoxystation (Don Whitley Scientific). DNA-PK inhibitor Nu7441 (10 µM, Medchem Express) was added at the time of seeding and replenished one hour before collecting the cells.
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