Cg tg k18 ace2 2prlmn j mice

Manufactured by Jackson ImmunoResearch

The Cg-Tg(K18-ACE2)2Prlmn/J mice are a transgenic mouse model that expresses the human angiotensin-converting enzyme 2 (hACE2) gene under the control of the keratin 18 (K18) promoter. The hACE2 gene is a known receptor for the SARS-CoV-2 virus, which causes COVID-19 in humans. These mice can be used to study the pathogenesis of SARS-CoV-2 infection and evaluate potential therapeutic interventions.

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Sars cov 2 isolate usa wa1 2020, by BEI Resources (1 mentions) K18 hace2 c57bl 6, by Jackson ImmunoResearch (1 mentions)

2 protocols using cg tg k18 ace2 2prlmn j mice

SARS-CoV-2 Infection Dynamics in K18-ACE2 Mice

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The Indiana University School approved all animal studies of the Institutional Animal Care and Use Committee (IACUC) and the Indiana University Institutional Biosafety Committee (IBC). Cg-Tg(K18-ACE2)2Prlmn/J mice (Jackson Laboratory, Bar Harbor, ME, Stock No: 034860) were used in this study. At approximately 8 weeks of age, K18-ACE2 mice were either mock infected (media only) or were infected (media containing virus) intranasally (20μl) with SARS-CoV-2 isolate USA-WA1/2020 (BEI Resources, Manassas, VA), n=4-5/group using methods described previously ^{10 (link),11 (link)}. Mice were administered with a viral concentration of 0 (mock) or 1x10⁴ (plaque-forming unit). As detailed below, several additional assessments were also completed each day, including body weight and body condition score (BCS). Criteria for humane endpoints were established in consultation with IACUC and collaborating veterinarians as described previously^{11 (link)}.
Discarded human specimens from COVID-19 patients were used to detect expression of ERK^1/2 phosphorylation. Use of human materials has been approved by Institutional Review Board (IRB).

Xie J., Klemsz M.J., Kacena M.A., Sandusky G., Zhang X, & Kaplan M.H. (2022). Inhibition of MEK signaling prevents SARS-CoV2-induced lung damage and improves survival of infected mice. Journal of medical virology, 94(12), 6097-6102.

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SARS-CoV-2 Infection in K18-hACE2 Mice

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All experiments were performed in accordance with animal protocols approved by the University of California, Irvine Institutional Animal Care and Use Committee. 8–16 week-old heterozygous K18-hACE2 C57BL/6 [strain: B6.Cg-Tg(K18-ACE2)2Prlmn/J] mice were obtained from Jackson Laboratory. Animals were housed by sex in single use disposable plastic cages and provided ad-libitum water. SARS-CoV-2 isolate USA-WA1/2020 was obtained from BEI. Mice were inoculated with between 10⁴-10⁵ PFU of SARS-CoV-2 in 10μL of DMEM or sham inoculated. Inoculations were performed under deep anesthesia through an intraperitoneal injection of a mixture of ketamine and xylazine. Infected and uninfected mice were examined and weighed daily. Animals were euthanized early if they reached pre-determined euthanasia criteria.

Olivarria G.M., Cheng Y., Furman S., Pachow C., Hohsfield L.A., Smith-Geater C., Miramontes R., Wu J., Burns M.S., Tsourmas K.I., Stocksdale J., Manlapaz C., Yong W.H., Teijaro J., Edwards R., Green K.N., Thompson L.M, & Lane T.E. (2021). Microglia do not restrict SARS-CoV-2 replication following infection of the central nervous system of K18-hACE2 transgenic mice. bioRxiv.

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