Vimentin antibodies 10366 1 ap

The sources of antibodies and reagents were: PTX3 antibodies #13797-1-AP (proteintech, China); Oct4 antibodies # 11263-1-AP (proteintech, China); Sox2 antibodies #11064-1-AP (Proteintech, China); Nanog antibodies #14295-1-AP (proteintech, China); E-cadherin antibodies #20874-1-AP (proteintech, China); N-cadherin antibodies #22018-1-AP (Proteintech, China); Snai1 antibodies #13099-1-AP (proteintech, China); Vimentin antibodies #10366-1-AP (Proteintech, China); Nav 1.5 antibody #23016-1-AP (Proteintech, China); NF-κB antibodies (Selleck, USA); p65/NF-κB # 10745-1-AP and p-p65 antibodies (Proteintech, China); IKKα and IκBα antibodies (Selleck, USA); pGSK3-β antibodies (Abcam, USA); GSK3-β antibodies (Abcam, USA); β-catenin antibodies # 51067-2-AP (proteintech, China); TNF-α (Proteintech, China); Peroxidase-conjugated goat anti-rabbit IgG (Proteintech, China); PRAP antibodies (Proteintech, China) and GAPDH antibodies (Proteintech, China). Human and mouse PTX3 ELISA kits (Boster Biological Technology, China); IKK-16, and Jingzhaotoxin-III (Tocris Bioscience, USA), rhPTX3 and siPTX3 (Guangzhou Ribobio, China) and Dimethyl sulfoxide (Beyotime Biotechnology, China).

Mouse-derived macrophage Raw264.7 was purchased from Shanghai Yuanzhixin Biotechnology Co (Shanghai, China). Mouse bone marrow-derived macrophages were primarily extracted from wild-type Balb/c mice, and the methods have been detailed previously [5 (link)]. Mouse aortic endothelial cells (MAECs) were purchased from Otwo Biotech Co (Shenzhen, China). CD68 antibodies (ab125212), inducible nitric oxide synthase (iNOS) antibodies (ab210823), CD31 antibodies (ab24590), α-SMA antibodies (ab21027), Collagen-I antibodies (ab260043), VE-Cadherin antibodies (ab33168), GADPH antibodies (ab9485), Alexa Fluor® 488-labeled donkey anti-mouse secondary antibody (ab150109), Alexa Fluor® 555-labeled donkey anti-rabbit secondary antibody (ab150062) were purchased from Abcam Co (Cambridge, MA, USA); Vimentin antibodies (10366-1-AP), F4/80 antibodies (28463-1-AP), CD206 antibodies (60143-1-Ig), Interferon-γ (IFN-γ) were purchased from Proteintech Co (Chicago, IL, USA); CD206 Cell Flow antibodies (17-2061-80), lipopolysaccharide (LPS; 00⁃4976⁃93) were purchased from Thermo Fisher Scientific Co (Waltham, MA, USA); iNOS Flow Cytology antibodies (130-116-421) was purchased from Miltenyi Biotec Co (Bergisch Gladbach, Germany); The primers were designed and synthesized by Nanjing DynaScience Biotechnology Co. TNF-α Antagonist III (R-7050; 303997-35-5) was purchased from Selleck. Co (Shanghai, China).