Imaging experiments were performed on an inverted Nikon Eclipse Ti microscope equipped with a PFS (perfect focus system), a Yokogawa CSU-X1 confocal spinning disk unit, two sCMOS Photometrics Prime 95B cameras for simultaneous dual acquisition to provide 95% quantum efficiency at 11 μm × 11 μm pixels and a Leica 100× oil objective (HC PL APO 1,4 oil immersion). We excited EGFP and IRFP with a 491 nm (100 mw) and a 635-nm laser (> 28 mW), respectively. We detected green and far red fluorescence with an emission filter using a 525/50 nm and a 708/75 nm detection window, respectively. A thermostated heater (Tokai Hit Stage Top Incubator) allowed for heating at 37 °C, humidity, and CO2 control (5%). Time-lapse analysis of GFP and IRFP foci was performed in 2D acquiring 241 time points at a 0.5 s time interval. The system was controlled using Metamorph 7.10 software. Time-lapse was concatenated into single TIFF file.
Prime 95b cameras
Manufactured by Leica
The Prime 95B is a high-performance camera designed for scientific and industrial applications. It features a large sensor, low noise, and high sensitivity, making it suitable for a variety of imaging tasks. The camera's core function is to capture detailed and accurate images for analysis and research purposes.
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Lab products found in correlation
2 protocols using prime 95b cameras
1
Dual-Color Live-Cell Imaging Microscopy
2
Simultaneous Dual-Color Live-Cell Imaging
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Imaging experiments were performed on an inverted Nikon Eclipse Ti microscope equipped with a PFS (perfect focus system), a Yokogawa CSU-X1 confocal spinning disk unit, two sCMOS Photometrics Prime 95B cameras for simultaneous dual acquisition to provide 95% quantum efficiency at 11 µm x 11 µm pixels and a Leica 100x oil objective (HC PL APO 1,4 oil immersion). We excited EGFP and IRFP with a 491 nm ( 100mw) and a 635-nm laser (>28mW),respectively. We detected green and far red fluorescence with an emission filter using a 525/50 nm and a 708/75 nm detection window, respectively. A thermostated heater (Tokai Hit Stage Top Incubator) allowed for heating at 37 o C, humidity and CO 2 control (5%). Time-lapse analysis of GFP and IRFP foci was performed in 2D acquiring 241 time points at a 0.5 s time interval. The system was controlled using Metamorph 7.10 software. Time-lapse was concatenated into single TIFF file.
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