MSC phenotype was assessed at passage 2 (p2) using the following monoclonal antibodies: anti-CD73-FITC (eBioscience®); anti-CD34-FITC (BD Biosciences®, BD); anti-CD45-PE (BD®); anti-CD90-PerCP-Cy5.5 (eBioscience®); anti-CD13-PE (eBioscience®); anti-CD11b -FITC (BD®) anti-CD44-APC and anti-HLA-DR-FITC (BD®). Staining of at least 50,000 cells was performed at 4°C for 15 min in the dark in presence of 1% human serum to avoid unspecific binding. Samples were acquired with a Gallios flow cytometer (Beckman Coulter®) and analyses were performed using FlowJo Software (BD®).
Anti cd13 pe
Manufactured by Thermo Fisher Scientific
Anti-CD13-PE is a fluorescent-labeled antibody that binds to the CD13 antigen on the surface of cells. CD13 is a cell surface metalloprotease enzyme expressed on myeloid cells, including monocytes, granulocytes, and their progenitors. This antibody can be used for the identification and enumeration of CD13-positive cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd44 apc, by BD (1 mentions)
Anti cd45 pe, by BD (1 mentions)
Anti hla dr fitc, by BD (1 mentions)
Anti cd73 fitc, by Thermo Fisher Scientific (1 mentions)
Anti cd11b fitc, by BD (1 mentions)
Gallios flow cytometer, by Beckman Coulter (1 mentions)
Anti cd34 fitc, by BD (1 mentions)
Anti cd133 pe, by Thermo Fisher Scientific (1 mentions)
Anti cd44 fitc, by Thermo Fisher Scientific (1 mentions)
Facscalibur, by BD (1 mentions)
Anti epcam pe, by Thermo Fisher Scientific (1 mentions)
Anti cd90 fitc, by Thermo Fisher Scientific (1 mentions)
Anti cd24 fitc, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti cd13 pe
1
Mesenchymal Stem Cell Phenotyping
2
Flow Cytometric Analysis of Cell Markers
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The following antibodies were used for flow cytometric analysis of cells: anti-CD24-FITC, anti-CD44-FITC, anti-CD90-FITC, anti-CD133-PE, anti-MDR1-PE, anti-EpCAM-PE, anti-CD13-PE (all from eBioscience, San Diego, CA) and anti-CDCP1-FITC and anti-ABCG2-PE (both from BioLegend, San Diego, CA). Cells were incubated with the indicated antibodies for 60 min and were then washed twice with PBS containing 2% FCS. Flow cytometric analysis was performed using a FACS-Calibur (BD Immunocytometry Systems, Franklin Lakes, NJ).
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