Nanoscope measurement acquisition software

AFM experiments were performed on a Bioscope Resolve BioAFM using a MLCT D triangular probe tip at 37 °C (Bruker). Spring constants of cantilever tips measured 0.03–0.08 N/m and were calibrated with nanoscope measurement acquisition software (Bruker). Samples were incubated in media containing 0.1% DMSO or escalating doses of cytochalasin B for 4 h prior to the experiment with an additional 30 min of system equilibration with the cantilever submerged. The cantilever was calibrated using nanoscope measurement acquisition software (Bruker). We analyzed force curves by finding the slope of the linear region of the curve measured during cantilever retraction of interphase cells in order to eliminate artifacts from pushing cells against the culture plate. This corresponded to the region from 20% to 80% of the maximum applied force on the cell (Fig. S7). Viscosity measurements were extracted from force curve data by calculating the area between the extended and retraction force curves as performed in Rebelo et al.^{23 (link)} (Fig. S7, shaded region).