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Op46 100ug

Manufactured by Merck Group
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Sourced in United Kingdom

The OP46-100UG is a laboratory equipment product from Merck Group. It serves as a core functional device for research and analytical applications. The details of its specific capabilities and intended use are not available in this factual, unbiased description.

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Lab products found in correlation

Ncl l p53 do7, by Leica (2 mentions) T6074, by Merck Group (1 mentions) Enhanced chemiluminescence, by GE Healthcare (1 mentions) Phospho p53 ser15, by Cell Signaling Technology (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions) X ray film, by Fujifilm (1 mentions) Cleaved caspase 3, by New England Biolabs (1 mentions)

2 protocols using op46 100ug

1

Nutlin-3 Treatment Protocol

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Cells were treated with Nutlin-3 at indicated doses for 4 h before cells lysates were harvested. Primary antibodies: mouse anti-MDM2 (1:300, # OP46-100UG, Merck Millipore), mouse anti-p21WAF1 1:100 (#: OP64, Merck Millipore), mouse anti-p53 (1:500, # NCL-L-p53-DO7, Leica Microsystems Ltd., Newcastle upon Tyne, UK) and mouse anti-α-tubulin (1:20,000, # T6074, Sigma) as a loading control (Figure S2).
Bradbury A., O’Donnell R., Drew Y., Curtin N.J, & Sharma Saha S. (2020). Characterisation of Ovarian Cancer Cell Line NIH-OVCAR3 and Implications of Genomic, Transcriptomic, Proteomic and Functional DNA Damage Response Biomarkers for Therapeutic Targeting. Cancers, 12(7), 1939.
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2

Western Blotting of Cell Signaling Proteins

Cited 1 time
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Western blotting was carried out as described in (31 (link)). Antibodies used were MDM2 (Ab-1) 1:300 (#: OP46-100UG, Merck Millipore), MDMX (#: A300287A-2 Bethyl laboratories), WIP1 (F-10) 1:200 (#: sc-376257, Santa Cruz Biotechnology), p53 1:500 (#: NCL-L-p53-DO7, Leica Microsystems Ltd.), phospho-p53Ser-15 1:1000 (#: 9284 Cell Signalling), p21WAF1 1:100 (#: OP64, Calbiochem), BAX 1:1000 (#: 2772S, Cell Signalling), cleaved caspase-3 1:1000 (#: 9664S, New England Biolabs Ltd.), actin 1:3000 (#: A4700, Sigma-Aldrich). Secondary goat anti-mouse/rabbit HRP-conjugated antibodies (#: P0447/P0448, Dako) were used at 1:1000. All antibodies were diluted in 5% milk/1 × TBS-tween (w/v). Proteins were visualised using enhanced chemiluminescence (GE Life Sciences) and X-ray film (Fujifilm). Densitometry was carried out using ImageJ software.
Esfandiari A., Hawthorne T.A., Nakjang S, & Lunec J. (2016). Chemical inhibition of wild-type p53 induced phosphatase 1 (WIP1/PPM1D) by GSK2830371 potentiates the sensitivity to MDM2 inhibitors in a p53-dependent manner. Molecular cancer therapeutics, 15(3), 379-391.
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