Vh032amine

VH032
amine and VH032-PEG4-amine hydrochloride
salt were purchased from MedChemExpress, LLC (Monmouth Junction, NJ).
BODIPY FL propionic acid was purchased from BroadPharm (San Diego,
CA). Acetic anhydride, DIPEA, DCM, DMSO, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide,
hydroxybenzotriazole, and all other basic chemical reagents and solvents
were purchased from Sigma-Aldrich (St. Louis, MO). DMSO-d₆ and chloroform-d were purchased from
Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). Reported protocols^{23 (link)} were adopted to assess or verify the reaction
progress and product purity and identity; to determine high-resolution
mass spectra; and to record ¹H and ¹³C NMR spectra
(Figures S1–S6).

The Tb-anti-GST antibody, 1,4-dithiothreitol
(DTT, 1 M), Tris (1 M, pH 7.5), and DMSO were purchased from Fisher
Scientific (Pittsburgh, PA). HEPES (1 M, pH 7.4) was purchased from
Teknova, Inc. (Hollister, CA). Triton X-100, Tween-20, and bovine
serum albumin were purchased from Sigma-Aldrich (St. Louis, MO). VH-298,
VH032-amine, Me-VH032-amine, and VH032-PEG4-NH₂ were purchased
from MedChemExpress, LLC (Monmouth Junction, NJ). BOC-VH032 was purchased
from LabNetwork (Cambridge, MA). VH032 phenol and thalidomide-4′-O-acetamido-alkylC4-amine were purchased from Bio-Techne
Corporation (Minneapolis, MN). MZ1, dBET1, and (+)-JQ1 were purchased
from Cayman Chemical (Ann Arbor, MI). Echo 384-well low dead volume
compound plates were purchased from Labcyte, Inc. (San Jose, CA).
384-well, black low-volume assay plates were purchased from Corning
Incorporated Life Sciences (Tewksbury, MA).