Prior to RNA-seq library preparation, ribosomal RNA was depleted from the total RNA using a Ribo-Zero rRNA Removal Kit for Gram-positive Bacteria (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. A Qubit RNA HS Assay and Agilent Bioanalyzer with an RNA 6000 Pico kit were used to assess the quantity and quality of the rRNA-depleted RNA samples. Six libraries (2 strains × 3 biological replicates) were constructed using a TruSeq Stranded mRNA Sample Preparation kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations. The libraries were sequenced on an Illumina Miseq platform using 300 base- length read chemistry in a paired-end mode.
Rna hs assay
Manufactured by Agilent Technologies
Sourced in United States
The RNA HS Assay is a high-sensitivity assay for quantification of RNA. It is designed to measure low concentrations of RNA samples with high accuracy and precision.
Automatically generated - may contain errors
Lab products found in correlation
Truseq stranded mrna sample preparation kit, by Illumina (1 mentions)
Miseq platform, by Illumina (1 mentions)
Bioanalyzer, by Agilent Technologies (1 mentions)
Rna 6000 pico kit, by Agilent Technologies (1 mentions)
Ribo zero rrna removal kit for gram positive bacteria, by Illumina (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions)
2 protocols using rna hs assay
1
Ribosomal RNA Depletion and RNA-seq
2
RNA Extraction from Cell Cultures
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The cells were cultured in 9 cm dishes. After humanteinine treatment for 48 h, the cells were washed twice in PBS, and Trizol reagent (Invitrogen, Carlsbad, CA, USA) was added to lyse cells. Total RNA was extracted, and the concentration and integrity were measured via Qubit RNA HS Assay and Agilent 2100 Bioanalyzer (Santa Clara, CA, USA), respectively.
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