Haematoxylin and eosin h e staining kit

Histopathological analysis of the collected tumour tissues was carried out using the Haematoxylin and Eosin (H&E) Staining Kit (#C0105S, Beyotime) according to the product instructions. Briefly, the tumour tissues were successively fixed in 4% PFA, dehydrated using gradient ethanol, embedded in paraffin, and sliced into 4 μm sections. The sections were then deparaffinized in xylene, hydrated in gradient ethanol, stained with haematoxylin for 2 min and eosin for 1 min. Finally, the sections were sealed with neutral resin. The results were observed and photographed with a microscope (30 (link)).

RSV, 2,3,5‐triphenyltetrazolium chloride (TTC) and dimethyl sulphoxide (DMSO) were obtained from Sigma Aldrich. The haematoxylin and eosin (H&E) staining kit (Cat#SBJ‐1247) and RIPA lysis buffer (#P0013B) were obtained from Beyotime Biotechnology. The primary antibodies used are as follows: p‐AMPK (#4188), AMPK (#5831), SIRT1(#9475), p‐FOXO1(#9461) FOXO1(#2880), LC3B (#2775), Beclin‐1 (#3495), Atg12(#4180), P62(#5114) were obtained from Cell Signaling Technology. β‐actin and β‐Tubulin from ZSGB‐BIO. EX527(HY‐15452) and Compound C (HY‐13418A) were obtained from MCE. HRP‐conjugated goat anti‐mouse/rabbit secondary antibodies came from ZSGB‐BIO. Prime Scripttr RT reagent Kit with gDNA Eraser (# AK3801) and SYBR Premix Ex TaqTMII (# AKA303) were from Takara. TNF‐α ELISA Kit (EK0527) and IL‐1β ELISA Kit (# EK0394) and were obtained from Wuhan Boster Biological Technology Ltd. LDH, SOD and MDA kits were purchased from Nanjing Jiancheng. Lipofectamine^TM2000 (Lip2000) was obtained from Invitrogen Life Technologies. Bromodeoxyuridine (Sigma). DAPI solution (#C1005, Beyotime).

Na₂WO₄·2H₂O, citric acid and glucose were purchased from Adamas-beta. Hydrochloric acid (37%) was obtained from Sinopharm. LuCl₃·6H₂O was purchased from Sigma‒Aldrich. Rhodamine B (RhB) was purchased from TCI. The aminophenyl fluorescein (APF) probe was purchased from AAT Bioquest. Phosphate buffered solution (PBS), Dulbecco’s modified Eagle medium (DMEM) and foetal bovine serum (FBS) were obtained from Gibco. Cell counting kit-8 (CCK-8), histone H₂AX rabbit polyclonal antibody, DAPI staining kit, annexin V-FITC apoptosis detection kit, haematoxylin and eosin (H&E) staining kit, NADH/NAD⁺ assay kit with WST-8 and TUNEL apoptosis assay kit were purchased from Beyotime.