Apoalert annexin 5 fitc apoptosis kit

Manufactured by BD

The BD ApoAlert annexin V-FITC Apoptosis Kit is a laboratory instrument designed for the detection and analysis of apoptosis, a programmed cell death process. The kit utilizes annexin V, a protein that binds to phosphatidylserine, and the fluorescent dye FITC to identify and quantify cells undergoing apoptosis.

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Lab products found in correlation

Facscalibur flow cytometer, by BD (1 mentions) Cellquest pro software, by BD (1 mentions) Synergy ht, by Agilent Technologies (1 mentions) Facscan system, by BD (1 mentions) Facscalibur system, by BD (1 mentions) Cellquest software, by BD (1 mentions) Cellquest software version 3, by BD (1 mentions) Modfit lt 3, by Verity Software House (1 mentions) Facsort, by BD (1 mentions)

Spelling variants of this product

Annexin V-FITC (1630 citations) Annexin V (870 citations) Annexin V-FITC kit (220 citations) Annexin V-FITC Apoptosis Kit (189 citations) Annexin V kit (54 citations) Annexin V Apoptosis Kit (43 citations) Annexin V (Annexin V-FITC) (5 citations) Apoptosis kits (4 citations) ApoAlert Annexin V-FITC kit (2 citations)

4 protocols using apoalert annexin 5 fitc apoptosis kit

Quantifying Apoptosis via Annexin V/PI Staining

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Quantification of apoptosis by Annexin V/propidium iodide (PI) staining was performed using a BD ApoAlert annexin V-FITC Apoptosis Kit (BD Biosciences, San Jose, CA) according to the manufacturer’s instructions. Both floating and attached cells were collected 24 h after drug treatment. The concentration of RAPA was 0.36 μM, and the concentration of cisplatin was 2.0 μM. The molar ratio of cisplatin to RAPA was 5.5. Cells were analyzed on a FACS Calibur flow cytometer using CellQuest Pro software (version 5.1.1; BD Biosciences, San Jose, CA).

Guo S., Lin C.M., Xu Z., Miao L., Wang Y, & Huang L. (2014). Co-delivery of Cisplatin and Rapamycin for Enhanced Anticancer Therapy through Synergistic Effects and Microenvironment Modulation. ACS Nano, 8(5), 4996-5009.

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Cell Viability and Apoptosis Assay

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For cell viability assay, 40,000 cells were plated per well in a 96-well plate and treated as indicated with various drug combinations for different time points. Then, the cells were treated with 200 μl of 0.6 mg/ml MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) in serum-free medium for 4 h, and then solubilized in dimethyl sulfoxide for 30 min and absorbance was measured with a spectrophotometer (Synergy HT; BioTek) at 595 nm. The percent of apoptotic cells was measured using a BD ApoAlert Annexin V-FITC apoptosis kit (BD Biosciences) according to the manufacturer’s instructions, and cells were analyzed on a FACscan system (Becton Dickinson). Additionally, cells were fixed, stained with antibodies against cleaved caspase 3 and cleaved PARP, and analyzed by flow cytometry.

Bagheri-Yarmand R., Sinha K.M., Li L., Lu Y., Cote G.J., Sherman S.I, & Gagel R.F. (2018). Combinations of Tyrosine Kinase Inhibitor and ERAD Inhibitor Promote Oxidative Stress-Induced Apoptosis through ATF4 and KLF9 in Medullary Thyroid Cancer. Molecular cancer research : MCR, 17(3), 751-760.

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Apoptosis Quantification in Cell Cultures

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Derivative cells and control cells were cultured to 80 % confluence. Cells were harvested after being washed three times with ice-cold phosphate-buffered saline (PBS). Subsequently, cells were labeled with Annexin V and propidium iodide according to the manufacturer’s protocol (BD ApoAlert Annexin V-FITC Apoptosis kit; BD Biosciences, PaloAlto, CA) [21 (link)]. The percentage of apoptotic cells was determined by (M2) peak in the histogram generated by FACSCalibur system and Cell Quest software (BD Biosciences).

Xiao X., Yang G., Bai P., Gui S., Nyuyen T.M., Mercado-Uribe I., Yang M., Zou J., Li Q., Xiao J., Chang B., Liu G., Wang H, & Liu J. (2016). Inhibition of nuclear factor-kappa B enhances the tumor growth of ovarian cancer cell line derived from a low-grade papillary serous carcinoma in p53-independent pathway. BMC Cancer, 16, 582.

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Apoptosis analysis of fisetin and micelles

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Flow cytometry was used to analyze the loss of membrane symmetry and membrane integrity using FITC Annexin V and propidium iodide (BD ApoAlertAnnexin V-FITC Apoptosis, kit, BD Biosciences), respectively. Briefly, cells were treated with fisetin and fisetin micelles at various concentrations (30 μmol/L、100 μmol/Land 300 μmol/L, respectively) for 48 h. Cells then were digested with non-EDTA trypsin for 3 min and harvested.5 × 10⁵Cells were labeled with propidium iodide (PI) and Annexin V-FITC for 30 min at room temperature. After filtration with a 300 dialyzer (FACSort, Becton Dickinson, San Jose, CA). Data were further analyzed by CellQuest software, version 3.1 (Becton Dickinson) and ModFit LT 3.2 (Verity Software House). Experiments were conducted in triplicate.

Xiao X., Zou J., Fang Y., Meng Y., Xiao C., Fu J., Liu S., Bai P, & Yao Y. (2018). Fisetin and polymeric micelles encapsulating fisetin exhibit potent cytotoxic effects towards ovarian cancer cells. BMC Complementary and Alternative Medicine, 18, 91.

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