Log In Sign up
The largest database of trusted experimental protocols

Mbl598

Manufactured by Merck Group
Visit Supplier

MBL598 is a laboratory centrifuge designed for general-purpose applications. It is capable of processing a range of sample volumes and can achieve high-speed separation of various materials. The centrifuge features programmable speed and time controls, allowing users to customize settings for their specific needs. Its compact design and intuitive user interface make it a versatile addition to many laboratory environments.

Automatically generated - may contain errors

Lab products found in correlation

Mab1637, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Bz x700, by Keyence (2 mentions) Sc 40, by Santa Cruz Biotechnology (1 mentions)

2 protocols using mbl598

1

Immunostaining of Neuronal Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cultured hippocampal neurons and Neuro 2a cells were fixed in 4% paraformaldehyde for 15 min at room temperature, and permeabilized in 0.3% Trinton-X100 for 10 min. Primary antibodies against GFP (MBL598; Merck; 1:500), Myc (sc-40; Santa Cruz Biotechnology; 1:200), cleaved caspase-3 (Asp175, Cell Signaling Technology, Inc; 1:800) andβIII tubulin (MAB1637; Merck; 1:500) were used. Nuclei were visualized with DAPI (Sigma). Imaging was performed using a light and fluorescence microscope (BZ-X700, KEYENCE).
Nguyen D.T., Le T.M., Hattori T., Takarada-Iemata M., Ishii H., Roboon J., Tamatani T., Kannon T., Hosomichi K., Tajima A., Taniuchi S., Miyake M., Oyadomari S., Tanaka T., Kato N., Saito S., Mori K, & Hori O. (2021). The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity. Scientific Reports, 11, 13086.
+ Open protocol
+ Expand
2

Immunostaining of Cultured Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cultured hippocampal neurons and Neuro 2a cells were xed in 4% paraformaldehyde for 15 min at room temperature, and permeabilized in 0.3% Trinton-X100 for 10 min. Primary antibodies against GFP (MBL598; Merck; 1:500), Myc (sc-40; Santa Cruz Biotechnology; 1:200), cleaved caspase-3 (Asp175, Cell Signaling Technology, Inc; 1:800) andβIII tubulin (MAB1637; Merck; 1:500) were used. Nuclei were visualized with DAPI (Sigma). Imaging was performed using a light and uorescence microscope (BZ-X700, KEYENCE).
Nguyen D.T., Le T.M., Hattori T., Takarada‐Iemata M., Ishii H., Roboon J., Tamatani T., Kannon T., Hosomichi K., Tajima A., Taniuchi S., Miyake M., Oyadomari S., Saito S., Mori K., & Hori O. (2021). The ATF6β-Calreticulin Axis Promotes Neuronal Survival Under Endoplasmic Reticulum Stress and Excitotoxicity.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!