Masson s trichrome stain kit

Manufactured by Bio-Optica

Sourced in Italy

Masson's trichrome stain kit is a set of reagents used for the histological staining of tissue samples. It is designed to differentiate collagen, muscle, and nuclei within the sample. The kit contains the necessary stains, such as Weigert's iron hematoxylin, Biebrich scarlet-acid fuchsin, and aniline blue, to achieve the distinctive coloring of the tissue components.

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Lab products found in correlation

Application suite x 2, by Leica (1 mentions) Provis ax70, by Olympus (1 mentions)

Spelling variants of this product

Masson’s trichrome (25 citations) Masson trichrome (19 citations) Masson’s trichrome stain (6 citations) Masson trichrome stain (3 citations)

2 protocols using masson s trichrome stain kit

Quantifying Cardiac ACE2 and Fibrosis

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IHC was performed on 4 μm FFPE human heart biopsies using anti-ACE2 antibody (cat. no. ab15348; Abcam, Cambridge, MA, USA; dilution, 1:200) and the HRP one-step polymer detection system: anti-mouse-rabbit-rat (ZyoChem Plus) and DAB. ACE2, Biopsies (n = 40) were analyzed and captured together under the same setting. ACE2 quantification was made according to user guidelines of imageJ. Tissue area was marked and the red channel threshold for all the biopsies was defined. The ACE2 + signal was calculated only within the cardiac tissue and normalized to the total area.
Masson’s trichrome stain kit (Bio-optica Milano, Italy) was used for fibrosis staining. We randomly selected 10 fields of heart sections at × 20 magnification by light microscopy and analyzed % era by ImageJ.

Herman-Edelstein M., Guetta T., Barnea A., Waldman M., Ben-Dor N., Barak Y., Kornowski R., Arad M., Hochhauser E, & Aravot D. (2021). Expression of the SARS-CoV-2 receptorACE2 in human heart is associated with uncontrolled diabetes, obesity, and activation of the renin angiotensin system. Cardiovascular Diabetology, 20, 90.

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Quantifying Cardiac Collagen Fibers

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Collagen fibers in hearts were detected by Masson's trichrome stain kit (Bio-Optica Milano S.p.a., Milan, Italy). Briefly, paraffin-embedded slices of hearts were deparaffined and trichrome stain was performed following the manufacturer's instruction. Collagen fibers were stained by aniline blue solution included in the kit. Slides were observed under optical microscope Olympus AX70 PROVIS (Olympus). Pictures were acquired by digital camera suite Leica Application Suite X 2.0.0.14332 (Leica Microsystems). The percentage of blue area vs total area was counted in ten randomly chosen fields at ×200 final magnification using ImageJ software.

Marano F., Frairia R., Rinella L., Argenziano M., Bussolati B., Grange C., Mastrocola R., Castellano I., Berta L., Cavalli R, & Catalano M.G. (2017). Combining doxorubicin-nanobubbles and shockwaves for anaplastic thyroid cancer treatment: preclinical study in a xenograft mouse model. Endocrine-related cancer, 24(6).

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