Indigo in vivo image software

For in vivo bioluminescent imaging, male Saa3-luc mice were injected intraperitoneally with D-luciferin (150 mg/kg body weight, Promega) and 10 minutes later, anesthetized with pentobarbital sodium. After 5 minutes, Saa3-luc mice were placed supine position on the plate and imaged for 1 min with the camera set at the highest sensitivity by NightOWL II Imaging Systems LB983 (Berthold Technologies, Bad Wildbad, Germany). Photons emitted from tissues were analyzed using Indigo in vivo image software (Berthold). Signal intensity was quantified as the sum of all detected photon counts per second and presented as count/sec (cps). For any given analysis, all images were adjusted to the same scale of minimum and maximum luminescent intensity.

Male Saa3 promoter-luc mice were injected intraperitoneally with D-luciferin (150 mg/kg body weight, Promega) and anesthetized with isoflurane. After 5 min, Saa3-luc mice were placed in a prone position on the plate and imaged for 1 min with the camera set to the highest sensitivity level using a NightOWL II Imaging Systems LB983 (Berthold Technologies, Bad Wildbad, Germany). Photons emitted from the tissues were analyzed using Indigo in vivo image software (Berthold). The signal intensity was quantified as the sum of all detected photon counts per second and presented as counts per second (cps)/mm². For any analysis, all images were adjusted to the same scale as the minimum and maximum luminescence intensities.

Male Saa3 promoter-luc mice were injected intraperitoneally with D-luciferin (150 mg/kg body weight, Promega) and then anesthetized with pentobarbital sodium (Kyoritsu Seiyaku, Tokyo, Japan). After five min, Saa3-luc mice were placed in a prone position on the plate and imaged for 1 min with the camera set at the highest sensitivity level by NightOWL II Imaging Systems LB983 (Berthold Technologies, Bad Wildbad, Germany), except the mice shown in Fig. 1e (they were placed in a supine position). Photons emitted from tissues were analyzed using Indigo in vivo image software (Berthold). Signal intensity was quantified as the sum of all detected photon counts per second and presented as count/sec (cps). For any given analysis, all images were adjusted to the same scale of minimum and maximum luminescent intensity.