Anti gapdh a300 641a

For Western blot analysis, protein samples were separated by SDS-PAGE, transferred to a nitrocellulose membrane (0.2 mm pore size; Bio-Rad, Hercules, CA, USA). The blots were probed with the primary antibodies listed below and bands were detected by horseradish peroxidase (HRP)-conjugated secondary antibody (Bio-Rad). Peroxidase activity was detected with the enhanced chemiluminescence detection method (WesternBright ECL, Advasta, Menlo Park, CA, USA). Primary antibodies used in this study were: rabbit polyclonal anti-ubiquitin antibody (kindly provided by Prof. A. L. Haas, Dept. of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, New Orleans); anti-HSF2 (sc-13056), anti-YY1 (sc-281), anti-specificity protein 1 (Sp1) (sc-420) and anti-specificity protein 3 (Sp3) (sc-644) from Santa Cruz Biotechnology (Dallas, TX, USA); anti-Lamin A/C (4C11) and anti-HSF1 (4356) from Cell Signaling Technology (Danvers, MA, USA); anti-GAPDH (A300-641A) from Bethyl Laboratories, Inc.; anti-actin (A 2066) from Sigma-Aldrich (Steinheim, Germany).