Supernatants of culture cells were harvested and concentrated 10 times using Amicon Ultra column 10kDa (EMD Millipore UFC501008). Rat IL-6, IL1-β and IL-18 cytokines were detected by Luminex assay (Bio-Rad Bio-plex Pro Cytokine).
Bio plex pro cytokine
Manufactured by Bio-Rad
Sourced in Germany
The Bio-plex Pro Cytokine is a multiplex immunoassay system developed by Bio-Rad. It allows for the simultaneous quantification of multiple cytokines, chemokines, and other soluble proteins in a single sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bio plex pro cytokine
1
Cytokine Profiling of Cultured Cells
2
Measuring IL-6 and IL-8 Inflammatory Markers
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To analyze the inflammatory response the interleukins 6 (IL-6) and 8 (IL-8) were measured by a multiplex bead assay (Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays, Bio Rad Laboratories GmbH, Feldkirchen, Germany). The measurements were performed according to the manufacturer’s instructions (21 ).
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