Ultivo qqq ms system

Oxylipin profiling was performed using UPLC with an Agilent Ultivo QQQ MS system coupled to an Agilent 1290 Infinity II UPLC system (Agilent, Santa Clara, CA, USA). Chromatographic separation of the oxylipins was achieved using a gradient of water, methanol, and acetonitrile, all with 0.1% acetic acid (v/v). The acquisition parameters were as previously described [32 (link)] with minor modifications, and the MS data were used for quantification. Surrogate analytes and internal and external standards were used to monitor extraction efficiency and ensure accurate quantitation with standard curves. The acquired data were quantified using Quant-My-Way (Agilent, Santa Clara, CA, USA) using 9 isotope-labeled internal standards. Here we report the data for oxylipins with >80% of values above the limit of detection (43 of 62 oxylipins) and for 4 PUFAs: arachidonic acid (ARA), linoleic acid (LA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) (Cayman Chemical, Ann Arbor, MI, USA). UPLC was performed in 2 separate batches, ensuring that repeat measures across time for the same participant were all included in the same batch.

The PGE₂, 5-HETE, 12-HETE, and 20-HETE quantification was performed on an Agilent Ultivo QQQ MS system coupled to an Agilent 1290 Infinity II UPLC system (Agilent, Santa Clara, CA, USA). Chromatographic separation of oxylipins was achieved using a gradient of water, methanol, and acetonitrile all with 0.1% acetic acid (v/v). Acquisition parameters were as previously described with minor modifications [20 (link)]. The acquired data were quantified by Quant-My-Way (Agilent, Santa Clara, CA, USA) using calibration curves.