Percp cyanine5.5 anti mouse cd206

The HES used in this study was obtained from Tokyo Chemical Industry (Tokyo, Japan), dissolved in distilled water, and administered orally at a dose of 5 or 10 mg/kg/day daily to mice.
For the flow cytometry analysis, FITC anti-mouse CD11b, AF647 anti-mouse F4/80, PE anti-mouse CD163, PerCP/Cyanine5.5 anti-mouse CD206, FITC anti-mouse CD45, and PE anti-mouse CD86 antibodies were purchased from BioLegend (San Diego, CA, USA). Specific antibodies against phospho-forkhead box O3a (p-FoxO3a), FoxO3a, phospho-AKT (p-AKT), AKT, phospho-mTOR (p-mTOR), mTOR, phospho-p70S6 kinase (p-p70S6K), and p70S6K were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against Fbx32, MuRF1, myostatin, and MyoD were purchased from Abcam (Cambridge, UK). Antibodies against phosphoinositide 3-kinase (PI3K), myogenin, and myocyte enhancer factor 2 (MEF-2) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

The antibodies used were anti-phospho-forkhead box O3 (p-FoxO3a), FoxO3a phospho-Akt (p-Akt), Akt, phospho-mammalian target of rapamycin (p-mTOR), mTOR (all purchased from Cell Signaling Technology, Danvers, MA, USA), F-box protein (Fbx32, also known as atrogin), muscle ring-finger 1 (MuRF1), myostatin, myoblast determination protein 1 (MyoD), type 2X-myosin heavy chain (MYH1) (all purchased from Abcam, Cambridge, UK), phosphoinositide 3-kinase (PI3K), myogenin, myocyte enhancer factor 2 (MEF-2), superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPx1), catalase (all purchased from Santa Cruz Biotechnology, Dallas, TX, USA), fluorescein isothiocyanate (FITC) anti-mouse CD11c, phycoerythrin (PE) anti-mouse CD45, PE anti-mouse CD86, and PerCP/Cyanine5.5 anti-mouse CD206 (all purchased from BioLegend, San Diego, CA, USA).