AmB analytical standard (87.8%), methanol (MeOH) high performance liquid chromatography (HPLC) grade, formic acid mass spectrometry grade, NaOH, MgCl 2 , CaCl 2 , CTAB, and NaHCO 3 were obtained from Sigma Aldrich (Germany); AmB API powder (85%) from Cayman Chemical (USA); BSA protease free powder fraction V, dimethyl sulfoxide (DMSO), dextrose, SLS, Na 2 HPO 4 , NaH 2 PO 4 , KH 2 PO 4 , NaCl and KCl from Fisher Scientific (USA); Tween 80 from Amresco (USA); GF/D (pore size 2.7 μm, 25 mm diameter) and GF/F (pore size 0.7 μm, 25 mm diameter) filters from Whatman (UK); regenerated cellulose (RC) filters 0.45 µm 13 mm diameter from Cronus (UK); cellulose ester dialysis tubing of 300 kDa MWCO from Spectrum Labs® (USA) and Sep -Pak® Vac 3cc (500 mg) tC18 SPE column from Waters (Massachusetts, USA).
Methanol meoh high performance liquid chromatography grade
Manufactured by Merck Group
Sourced in Germany
Methanol (MeOH) HPLC grade is a high-purity solvent commonly used as the mobile phase in high-performance liquid chromatography (HPLC) applications. It is designed to meet the stringent requirements for HPLC analysis, including low levels of impurities and contaminants.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Na2hpo4, by Thermo Fisher Scientific (3 mentions)
Mgcl2, by Merck Group (3 mentions)
Nah2po4, by Thermo Fisher Scientific (3 mentions)
Cacl2, by Merck Group (3 mentions)
Whatman, by Cytiva (3 mentions)
Mass spectrometry grade formic acid, by Merck Group (2 mentions)
Tween 80, by Avantor (2 mentions)
Amb api powder, by Cayman Chemical (2 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (2 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (2 mentions)
Nh4hco2, by Merck Group (2 mentions)
Nahco3, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Kh2po4, by Thermo Fisher Scientific (1 mentions)
3 protocols using methanol meoh high performance liquid chromatography grade
1
Analytical Characterization of Amphotericin B
2
Antifungal AmB Characterization Protocol
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AmB analytical standard (87.8%), Methanol (MeOH) High Performance Liquid Chromatography (HPLC) grade, formic acid, Sabouraud Dextrose (SBD) Broth, NaOH, MgCl2, CaCl2, NHCO3 and NH4HCO2 were obtained from Sigma Aldrich (Germany); AmB active pharmaceutical ingredient (API) powder (85%) from Cayman Chemical (USA); Bovine Serum Albumin Protease Free Powder Fraction V (BSA), dimethyl sulfoxide (DMSO), dextrose, Na2HPO4, NaH2PO4, NaCl and KCl were obtained from Fisher Scientific (USA); Sabouraud dextrose (SBD) agar was obtained from Oxoid (UK), 25 mL sterile universal culture tubes were obtained from Sterilin Thermo Scientific (UK); 10 μL plastic loops from Microspec (UK); GF/D (pore size 2.7 μm, 25 mm diameter) and GF/F (pore size 0.7 μm, 25 mm diameter) filters were obtained from Whatman (UK) and regenerated cellulose (RC) filters 0.45 µm 13 mm diameter from Cronus (UK).
The yeast strain used in the microbiology experiments was Candida albicans SC5314 [18] .
The yeast strain used in the microbiology experiments was Candida albicans SC5314 [18] .
3
Analytical Standard Characterization Protocol
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AmB analytical standard (87.8%), methanol (MeOH) high performance liquid chromatography (HPLC) grade, formic acid mass spectrometry grade, NaOH, MgCl2, CaCl2, hexadecyltrimethylammonium bromide (CTAB), cholesterol (CH), NHCO3 and NH4HCO2 were obtained from Sigma Aldrich (Germany); ethylenediaminetetracetic acid anhydrous (EDTA) from Sigma Aldrich (USA); AmB API powder (85%) from Cayman Chemical (USA); BSA protease free powder fraction V, dimethyl sulfoxide (DMSO), dextrose, sodium dodecyl sulphate (SLS), Na2HPO4, NaH2PO4, KH2PO4, NaCl and KCl from Fisher Scientific (USA); Tween 80 from Amresco (USA); phosphatidylcholine from egg from Lipoid GmbH (Germany); sodium taurocholate from Prodotti Chimici e Alimentaria (Italy); GF/D (pore size 2.7 μm, 25 mm diameter) and GF/F (pore size 0.7 μm, 25 mm diameter) filters from Whatman (UK) and regenerated cellulose (RC) filters 0.45 µm 13 mm diameter from Cronus (UK).
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