Chemicals: chemicals of this work (4-(N,N-dimethylaminobenzaldhyde, Cyanothioacetamide, Piperidine, Methyl iodide, Ethyl Chloroacetate, 2-Chloroacetamide, Chloroacetonitrile or N-aryl-2-Chloroacetamides, Ethanol, Sodium acetate.3H2O, Sodium carbonate) were purchased from Sigma Aldrich Co.
Instrumentations: Melting points were determined on a Gallan-Kamp apparatus and are uncorrected. The purity of the compounds was ensured by TLC and the spectroscopic analysis.
IR spectra were recorded on a Shimadzu 470 IR-spectrophotometer (KBr; νmax in cm−1). The 1H and 13C NMR spectra were recorded on Varian A5 500 MHz spectrometer using DMSO-d6 as a solvent and tetramethylsilane (TMS) as an internal reference. Coupling constants (J values) are given in Hertz (Hz). Elemental analyses were performed on a Perkin Elmer 2400 LS Series CHN/O analyzer.
Cell lines: The in vitro human breast cancerous cell line (MCF7), lung cancerous cell lines (A549) and normal cell lines were purchased from Serum and Vaccine formulation in Cairo-Egypt.
Molecular docking: Molecular docking studies were performed in (I Mole Lab for bioinformatics, Cairo, Egypt).
Softwares: The biological data was analyzed and plot by Graphpad prism, Cell qust, ANOVA, Origin Lab, AutoDock Vina 1.1.2, Mestrenova and Excel software.
Instrumentations: Melting points were determined on a Gallan-Kamp apparatus and are uncorrected. The purity of the compounds was ensured by TLC and the spectroscopic analysis.
IR spectra were recorded on a Shimadzu 470 IR-spectrophotometer (KBr; νmax in cm−1). The 1H and 13C NMR spectra were recorded on Varian A5 500 MHz spectrometer using DMSO-d6 as a solvent and tetramethylsilane (TMS) as an internal reference. Coupling constants (J values) are given in Hertz (Hz). Elemental analyses were performed on a Perkin Elmer 2400 LS Series CHN/O analyzer.
Cell lines: The in vitro human breast cancerous cell line (MCF7), lung cancerous cell lines (A549) and normal cell lines were purchased from Serum and Vaccine formulation in Cairo-Egypt.
Molecular docking: Molecular docking studies were performed in (I Mole Lab for bioinformatics, Cairo, Egypt).
Softwares: The biological data was analyzed and plot by Graphpad prism, Cell qust, ANOVA, Origin Lab, AutoDock Vina 1.1.2, Mestrenova and Excel software.