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Prussian blue stain

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Prussian blue stain is a laboratory reagent used for the identification and visualization of iron compounds in histological and cytological samples. It is a simple, cost-effective staining technique that provides a distinctive blue color when iron is present. The stain has a well-established utility in various areas of medical and biological research, but a detailed description of its intended use would require more context-specific information.

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Lab products found in correlation

Shandon cytospin 4 cytocentrifuge, by Thermo Fisher Scientific (2 mentions) May grunwald giemsa stain, by Merck Group (1 mentions) May gr nwald giemsa stain, by Merck Group (1 mentions)

2 protocols using prussian blue stain

1

Staining and Quantification of Ring Sideroblasts

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Cells were centrifuged at 500 rpm for 3 min using the Shandon Cytospin 4 Cytocentrifuge (Thermo Fisher Scientific). The cells were subsequently stained with May-Grunwald Giemsa stain (Merck KGaA, Darmstadt, Germany) and Prussian blue stain (Sigma-Aldrich). Ring sideroblasts were defined as erythroblasts wherein 5 or more siderotic granules cover at least one-third of the nucleus circumference, as per the recommendation by the International Working Group on Morphology of Myelodysplastic Syndrome58 (link).
Ono K., Fujiwara T., Saito K., Nishizawa H., Takahashi N., Suzuki C., Ochi T., Kato H., Ishii Y., Onodera K., Ichikawa S., Fukuhara N., Onishi Y., Yokoyama H., Yamada R., Nakamura Y., Igarashi K, & Harigae H. (2022). Congenital sideroblastic anemia model due to ALAS2 mutation is susceptible to ferroptosis. Scientific Reports, 12, 9024.
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2

Quantification of Sideroblasts in Erythroblasts

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Freshly-isolated Ter119-positive cells were centrifuged at 500 rpm for 3 min using the Shandon Cytospin 4 cytocentrifuge (Thermo Fisher Scientific). The cells were subsequently stained with May-Grünwald Giemsa stain (Merck KGaA, Darmstadt, Germany) and Prussian blue stain (Sigma-Aldrich Corp.). The frequency of sideroblasts in 500 erythroblasts in BM was quantified by light microscopy.
Inokura K., Fujiwara T., Saito K., Iino T., Hatta S., Okitsu Y., Fukuhara N., Onishi Y., Ishizawa K., Shimoda K, & Harigae H. (2017). Impact of TET2 deficiency on iron metabolism in erythroblasts. Experimental hematology, 49.
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