Isoamyl alcohol

Ethylene diamine tetraacetic acid (EDTA), cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), Tris-HCl, NaCl, urea, Na₂CO₃, NaHCO₃, sodium citrate (C₆H₅Na₃O₇), guanidine isothiocyanate (C₂H₆N₄S), sodium N-dodecanoylsalcosinate (C₁₅H₁₀NO₄Na), β-mercaptoethanol, alcohol, isoamyl alcohol, and isopropanol were purchased from Sangon Biotech (Shanghai, China). HAuCl₄ was purchased from Sinopharm (Shanghai, China). Bovine serum albumin (BSA), sucrose, and alginate were obtained from Meilunbio Biotech (Dalian, China). Absorbent pads (CH27) and sample pads (SB06) were purchased from KinBio Biotech (Shanghai, China). The gold absorbent pad (GL0194), PVC backing plate (DB-6), and nitrocellulose membrane (Millipore 135) were obtained from Jiyi Biotech (Shanghai, China). PCR Master Max and the anti-5-FAM antibody were obtained from BBI Biotech (Shanghai, China). Streptavidin and biotin-BSA were obtained from Solarbio Biotech (Beijing, China).

Antennae from twenty moths were homogenized in 1 mL Trizol (Invitrogen, Carlsbad, California, USA) and RNA was extracted following the manufacturer’s instructions. The RNA was treated with RQ1 DNase (Promega, Madison, USA) to avoid the risk of genomic DNA contamination, followed by purification with phenol, chloroform and isoamyl alcohol (Sangon, Shanghai, China). The quality of RNA was evaluated by agarose gel electrophoresis and its concentration measured with Nanodrop 2000 (NanoDrop Technologies, Wilmington, DE, USA). The first strand cDNA was synthesized from 2 μg RNA utilizing cDNA synthesis with SuperScript® III RT kit (Invitrogen).